CAJ | 학술논문

目的：研究促红细胞生成素（EPO）促进血管性痴呆（VD）大鼠神经干细胞（NSC）增殖的分子调控机制。方法选取 SD 大鼠60只，采用改良血管阻断加硝普钠降压法建立 VD 大鼠模型，随机分为 VD 组和治疗组；治疗组腹腔注射EPO，分别于建模后7、14 d 提取分离各组大鼠脑组织 RNA，采用 RT-PCR 检测 NSC 增殖相关调控基因 FGF2、EGF、TGFа表达。结果治疗组7 d、14 d 时的 FGF2、EGF 表达均高于 VD 组（P <0.05），TGFа表达两组无统计学差异；7 d 时两组 FGF2表达明显高于 EGF（P <0.05），14 d 时则无统计学差异。结论 EPO 通过上调胞外 FGF2及 EGF 表达促进 VD 大鼠 NSC 增殖；在 VD大鼠 NSC 增殖早期，FGF2起主要作用。
목적：연구촉홍세포생성소（EPO）촉진혈관성치태（VD）대서신경간세포（NSC）증식적분자조공궤제。방법선취 SD 대서60지，채용개량혈관조단가초보납강압법건립 VD 대서모형，수궤분위 VD 조화치료조；치료조복강주사EPO，분별우건모후7、14 d 제취분리각조대서뇌조직 RNA，채용 RT-PCR 검측 NSC 증식상관조공기인 FGF2、EGF、TGFа표체。결과치료조7 d、14 d 시적 FGF2、EGF 표체균고우 VD 조（P <0.05），TGFа표체량조무통계학차이；7 d 시량조 FGF2표체명현고우 EGF（P <0.05），14 d 시칙무통계학차이。결론 EPO 통과상조포외 FGF2급 EGF 표체촉진 VD 대서 NSC 증식；재 VD대서 NSC 증식조기，FGF2기주요작용。
Objective To explore the molecular mechanism of erythropoietin(EPO)in promoting the proliferation of neural stem cells( NSC)in rats with vascular dementia( VD). Methods 60 SD rats were selected and the modified vessel occlusion and depressurization with sodium nitroprusside were applied to the establishment of VD rat models;the 60 VD rats were randomly divided into 2 groups:VD group and treatment group;intraperitoneal injection of EPO was made to rats in treatment group,while the same amount of saline to rats in VD group;RNA isolated from rat brain tissue was extracted at the time point of 7 days and 14 days after the model establishment;RT-PCR was applied to detect the expression of FGF2,EGF and TGFa,the regulatory genes related to NSC proliferation. Results The expressions of FGF2 and EGF in treatment group at the time point of 7 days and 14 dyas after the model establishment were higher than those in VD group(P <0. 05),while there existed no statistical difference in the expression of TGFa between the 2 groups;the expression of FGF2 was higher than that of EGF at the time point of 7 days after the model establishment in both groups,while there existed no statistical difference between the expression of FGF2 and that of EGF at the time point of 14 days after the model establishment in both groups( P < 0. 05). Conclusions EPO promotes the proliferation of NSC in VD rats by up-regulating the expressions of extracellular FGF2 and EGF;FGF2 plays a main role in the early proliferation of NSC in VD rats.