临床口腔医学杂志
臨床口腔醫學雜誌
림상구강의학잡지
JOURNAL OF CLINICAL STOMATOLOGY
2014年
10期
606-609
,共4页
口腔黏膜下纤维性变%槟榔碱%AngⅡ%氯沙坦%α-SMA%肌成纤维细胞%内皮-间充质转化
口腔黏膜下纖維性變%檳榔堿%AngⅡ%氯沙坦%α-SMA%肌成纖維細胞%內皮-間充質轉化
구강점막하섬유성변%빈랑감%AngⅡ%록사탄%α-SMA%기성섬유세포%내피-간충질전화
oral submucous fibrosis%arecoline%angiotensinⅡ%losartan%alpha-smooth muscle actin,myofibroblast%endothelial-to-mesenchymal transition
目的:检测氯沙坦干预下、槟榔碱诱导的人脐静脉内皮细胞(HUVECs)表达α-SMA的情况。方法:用不同浓度槟榔碱刺激HUVECs,采用免疫细胞印迹法检测各组HUVECs中α-SMA的表达。再以固定槟榔碱浓度诱导HUVECs,不同浓度氯沙坦干预,检测各组HUVECs中α-SMA的表达。结果:槟榔碱可诱导HUVECs表达α-SMA,经氯沙坦干预后α-SMA的表达受到一定抑制。结论:HUVECs经槟榔碱刺激后可转化为表达α-SMA的肌成纤维细胞。血管紧张素Ⅱ受体拮抗剂氯沙坦可部分抑制这种转化,提示血管紧张素Ⅱ及其受体参与了槟榔碱诱导的内皮-间充质转化。
目的:檢測氯沙坦榦預下、檳榔堿誘導的人臍靜脈內皮細胞(HUVECs)錶達α-SMA的情況。方法:用不同濃度檳榔堿刺激HUVECs,採用免疫細胞印跡法檢測各組HUVECs中α-SMA的錶達。再以固定檳榔堿濃度誘導HUVECs,不同濃度氯沙坦榦預,檢測各組HUVECs中α-SMA的錶達。結果:檳榔堿可誘導HUVECs錶達α-SMA,經氯沙坦榦預後α-SMA的錶達受到一定抑製。結論:HUVECs經檳榔堿刺激後可轉化為錶達α-SMA的肌成纖維細胞。血管緊張素Ⅱ受體拮抗劑氯沙坦可部分抑製這種轉化,提示血管緊張素Ⅱ及其受體參與瞭檳榔堿誘導的內皮-間充質轉化。
목적:검측록사탄간예하、빈랑감유도적인제정맥내피세포(HUVECs)표체α-SMA적정황。방법:용불동농도빈랑감자격HUVECs,채용면역세포인적법검측각조HUVECs중α-SMA적표체。재이고정빈랑감농도유도HUVECs,불동농도록사탄간예,검측각조HUVECs중α-SMA적표체。결과:빈랑감가유도HUVECs표체α-SMA,경록사탄간예후α-SMA적표체수도일정억제。결론:HUVECs경빈랑감자격후가전화위표체α-SMA적기성섬유세포。혈관긴장소Ⅱ수체길항제록사탄가부분억제저충전화,제시혈관긴장소Ⅱ급기수체삼여료빈랑감유도적내피-간충질전화。
Objective:To test how losartan inhibits the expression of α-SMA protein in Human Umbilical Vein Endothelial Cells (HUVECs),which stimulated by arecoline. Method:With different concentrations of arecoline stimulate HUVECs,respectively,detected the expression of α-SMA in HUVECs using the method of Western Blot. Then With certain concentrations of arecoline induce HUVECs,making different concentrations of losartan stimulate HUVECs,respectively, detected the expression of α-SMA in HUVECs. Result:HUVECs can express α-SMA induced by Arecoline, and the the expression of α-SMA can be inhibited after the stimulation of losartan. Conclusion:HUVECs stimulated by arecoline transformed into myofibroblast expressing the α-SMA. And AT1R inhibitor can inhibit this transformation to some extent, which suggesting that AngⅡand AT1R may play roles in the endothelial-to-mesenchymal transition induced by arecoline.