CAJ | 학술논문

目的：探讨硫代乙酰胺（TAA）诱导的 A 型肝性脑病小鼠的精神障碍。方法30只雄性昆明种小鼠随机分为实验组15只，对照组15只。实验组采用 TAA 200 mg·kg -1·d -1，连续腹腔注射3 d 至累积计量到600 mg/kg，正常对照组腹腔注射生理盐水，造模24 h 后进行脑功能评分、明暗箱、旷场及高架十字实验。行为学检测结束后24 h 行血氨及肝脏生化指标检测。两组间比较采用独立样本 t 检验。结果实验组小鼠在明暗箱实验中的明箱滞留时间（t ＝-4．006，P ＜0．01）与穿梭次数（t ＝-2．656，P ＜0．05）；旷场实验的中央活动路程（t ＝-3．639，P ＜0．05）、中央活动时间（t ＝-2．294，P ＜0．05）、垂直运动次数（t ＝-2．282，P ＜0.05）、理毛时间（t ＝5．992，P ＜0．01）；高架十字实验的开臂进入次数（t ＝-3．584，P ＜0．05）、开臂滞留时间（t ＝-3．992，P ＜0.05）与对照组小鼠相比，差异均具有统计学意义。但旷场实验中两组小鼠活动总路程差异无统计学意义（t ＝-0．96，P ＞0．05）。且实验组小鼠血氨（t ＝-3．168，P ＜0．05）、ALT（t ＝4．316，P ＜0．05）、AST（t ＝-2．581，P ＜0．05）、TBil（t ＝-9．127，P ＜0．01）水平明显大于对照组，差异均有统计学意义。结论用 TAA 腹腔注射建立的 A 型肝性脑病小鼠模型有焦虑样反应；小剂量 TAA 诱导A 型肝性脑病小鼠的焦虑样反应不伴有自发活动能力下降。
목적：탐토류대을선알（TAA）유도적 A 형간성뇌병소서적정신장애。방법30지웅성곤명충소서수궤분위실험조15지，대조조15지。실험조채용 TAA 200 mg·kg -1·d -1，련속복강주사3 d 지루적계량도600 mg/kg，정상대조조복강주사생리염수，조모24 h 후진행뇌공능평분、명암상、광장급고가십자실험。행위학검측결속후24 h 행혈안급간장생화지표검측。량조간비교채용독립양본 t 검험。결과실험조소서재명암상실험중적명상체류시간（t ＝-4．006，P ＜0．01）여천사차수（t ＝-2．656，P ＜0．05）；광장실험적중앙활동로정（t ＝-3．639，P ＜0．05）、중앙활동시간（t ＝-2．294，P ＜0．05）、수직운동차수（t ＝-2．282，P ＜0.05）、리모시간（t ＝5．992，P ＜0．01）；고가십자실험적개비진입차수（t ＝-3．584，P ＜0．05）、개비체류시간（t ＝-3．992，P ＜0.05）여대조조소서상비，차이균구유통계학의의。단광장실험중량조소서활동총로정차이무통계학의의（t ＝-0．96，P ＞0．05）。차실험조소서혈안（t ＝-3．168，P ＜0．05）、ALT（t ＝4．316，P ＜0．05）、AST（t ＝-2．581，P ＜0．05）、TBil（t ＝-9．127，P ＜0．01）수평명현대우대조조，차이균유통계학의의。결론용 TAA 복강주사건립적 A 형간성뇌병소서모형유초필양반응；소제량 TAA 유도A 형간성뇌병소서적초필양반응불반유자발활동능력하강。
Objective To evaluate the mental disorders in mice with thioacetamide (TAA)-induced acute hepatic encephalopathy (AHE). Methods Thirty male Kunming mice were equally and randomly divided into two groups (treatment group and control group).Mice in the treatment group received an intraperitoneal injection of TAA (200 mg·kg -1 ·d -1 for 3 days,up to 600 mg·kg -1 ),as compared with the same volume of physiological saline in the control group.Neurological function score,light/dark box,open field,and elevated plus -maze test were used to determine behavioral parameters at 24 h after the model was established.Serum ammonia,aspartate amino transferase (AST),al-anine aminotransferase (ALT),and total bilirubin (TBil)were measured at 24 h post determination of behavioral parameters.Comparison be-tween two groups was made by independent -samples t test.Results In the light/dark box test,the treatment group had significantly reduced residence time in the light box and number of shuttles between two boxes (t =-4.006,P <0.01;t =-2.656,P <0.05);in the open field test,the treatment group had significantly reduced central distance,central time,and number of vertical movements (t =-3.639,P <0.05;t =-2.294,P <0.05;t =-2.282,P <0.05),as well as increased grooming time (t =5.992,P <0.01);meanwhile,the number of open-arm entries and residence time in the open arm for the treatment group were also reduced in the elevated plus -maze test,as compared with the control group (t =-3.584,P <0.05;t =-3.992,P <0.05).However,the total distance in the open field test showed no significant difference between the two groups (t =-0.96,P >0.05).Moreover,compared with the control group,the treatment group had significant-ly higher levels of serum ammonia (t =-3.168,P <0.05),ALT (t =4.316,P <0.05),AST (t =-2.581,P <0.05),and TBil (t =-9.127,P <0.01).Conclusion Mice with AHE induced by an intraperitoneal injection of low -dose TAA have anxiety -related behav-iors;the anxiety -related behaviors are not associated with reduced locomotor activities.