中华现代护理杂志
中華現代護理雜誌
중화현대호리잡지
CHINESE JOURNAL OF MODERN NURSING
2014年
14期
1739-1741
,共3页
内窥镜%清洗消毒%细菌培养法%ATP荧光检测法
內窺鏡%清洗消毒%細菌培養法%ATP熒光檢測法
내규경%청세소독%세균배양법%ATP형광검측법
Gastroscope%Cleaning and disinfection%Bacterial counting%ATP bioluminesece detectiom
目的：通过细菌培养法和ATP荧光检测法监测和改进内镜清洗的环节，提高内镜清洗效果，有效保证内镜清洗消毒的效果。方法将60条使用后的内镜按照随机数字表分为对照组和观察组，每组各30条。对照组的清洗方法为常规床旁预处理、床旁负压吸引的压力、酶洗液的温度为常温；观察组的清洗方法为改进床旁预处理、增加床旁负压吸引的压力、提高酶洗液的温度。通过两种常用内镜微生物学监测方法评价两组内镜清洗前及清洗后内镜活检管腔的细菌残留情况。结果两组内镜清洗前内镜活检管腔中细菌计数和ATP荧光读数均值，差异无统计学意义（P＞0．05）；清洗后，观察组内镜活检管腔中细菌计数和ATP荧光读数均值分别为0 cfu，（210±32）RLU，低于对照组的（32±17）cfu，（4940±256）RLU，差异有统计学意义（t值分别为3．090，2．391；P＜0．05）。结论内镜清洗环节十分重要，有效清洗能够显著地降低内镜微生物水平，保证内镜清洗消毒。
目的：通過細菌培養法和ATP熒光檢測法鑑測和改進內鏡清洗的環節，提高內鏡清洗效果，有效保證內鏡清洗消毒的效果。方法將60條使用後的內鏡按照隨機數字錶分為對照組和觀察組，每組各30條。對照組的清洗方法為常規床徬預處理、床徬負壓吸引的壓力、酶洗液的溫度為常溫；觀察組的清洗方法為改進床徬預處理、增加床徬負壓吸引的壓力、提高酶洗液的溫度。通過兩種常用內鏡微生物學鑑測方法評價兩組內鏡清洗前及清洗後內鏡活檢管腔的細菌殘留情況。結果兩組內鏡清洗前內鏡活檢管腔中細菌計數和ATP熒光讀數均值，差異無統計學意義（P＞0．05）；清洗後，觀察組內鏡活檢管腔中細菌計數和ATP熒光讀數均值分彆為0 cfu，（210±32）RLU，低于對照組的（32±17）cfu，（4940±256）RLU，差異有統計學意義（t值分彆為3．090，2．391；P＜0．05）。結論內鏡清洗環節十分重要，有效清洗能夠顯著地降低內鏡微生物水平，保證內鏡清洗消毒。
목적：통과세균배양법화ATP형광검측법감측화개진내경청세적배절，제고내경청세효과，유효보증내경청세소독적효과。방법장60조사용후적내경안조수궤수자표분위대조조화관찰조，매조각30조。대조조적청세방법위상규상방예처리、상방부압흡인적압력、매세액적온도위상온；관찰조적청세방법위개진상방예처리、증가상방부압흡인적압력、제고매세액적온도。통과량충상용내경미생물학감측방법평개량조내경청세전급청세후내경활검관강적세균잔류정황。결과량조내경청세전내경활검관강중세균계수화ATP형광독수균치，차이무통계학의의（P＞0．05）；청세후，관찰조내경활검관강중세균계수화ATP형광독수균치분별위0 cfu，（210±32）RLU，저우대조조적（32±17）cfu，（4940±256）RLU，차이유통계학의의（t치분별위3．090，2．391；P＜0．05）。결론내경청세배절십분중요，유효청세능구현저지강저내경미생물수평，보증내경청세소독。
Objective Monitoring and evolving the cleaning process of patient-used gastroscope by the method of Surviving Bacterial counting and ATP bioluminesece detection , in order to improve the cleaning effect and insure the success of endoscope reprocessing .Methods A total of 60 clinical used gastroscopes were randomly divided into the control group ( 30 scopes ) and the test group ( 30 scopes ) .Two kinds of testing methods was used to study the microorganism residue in the biopsy channel of patient -used gastroscope before and after normal manual cleaning .After manual cleaning process was improved , then measure the microorganism residue of gastroscope again and compared with the normal manual cleaning method .Results The results of the Bacterial counting and the ATP bioluminesece detection of patient-used gastroscope were significantly different (P>0.05).After the cleaning process, the results of bacteria count and ATP bioluminesece were 0 cfu/scope and (210 ±32) RLU/scope, which were significantly lower than (32 ±17) cfu/scope and (4 940 ±256) RLU/scope.The differences were statistically different (t=3.090,2.391, respectivley;P<0.05).Conclusions The process of endoscope cleaning is important and can significantly reduce the microorganism level of patient -used endoscope , so it qualified the disinfection of endoscope .
