CAJ | 학술논문

目的：分析半胱氨酸蛋白酶8（CASP 8）基因-6526N I/D 多态性与2型糖尿病（T2DM）易感性的关系。方法：采用PCR-RFLP技术结合DNA测序检测414例正常人和410例2型糖尿患者 CASP 8基因-6526N I/D 的多态性。结果：对照组和糖尿病组I/I、I/D和 D/D的基因型频率分别为56．5％、38．9％、4．6％和58．0％、32．9％、9．0％，差异有统计学意义（P＜0．05）。D/D基因型发生T2DM的风险为I/I基因型的1．916倍（95％CI＝1．199～3．054，P＜0．05）。 D/D 基因型的空腹血糖高于I/D和I/I基因型（P＜0．05）。结论：CASP 8基因-6526N I/D多态性与2型糖尿病的发生相关。
목적：분석반광안산단백매8（CASP 8）기인-6526N I/D 다태성여2형당뇨병（T2DM）역감성적관계。방법：채용PCR-RFLP기술결합DNA측서검측414례정상인화410례2형당뇨환자 CASP 8기인-6526N I/D 적다태성。결과：대조조화당뇨병조I/I、I/D화 D/D적기인형빈솔분별위56．5％、38．9％、4．6％화58．0％、32．9％、9．0％，차이유통계학의의（P＜0．05）。D/D기인형발생T2DM적풍험위I/I기인형적1．916배（95％CI＝1．199～3．054，P＜0．05）。 D/D 기인형적공복혈당고우I/D화I/I기인형（P＜0．05）。결론：CASP 8기인-6526N I/D다태성여2형당뇨병적발생상관。
Objective To investigate the association of cysteine aspartic acid specific protease 8 (CASP 8) gene-652 6N Insertion/Deletion polymorphisms and susceptibility of type 2 diabetes mellitus (T2DM). Methods CASP 8 gene -652 6N I/D polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing in 414 controls and 410 patients with T2DM. Results I/I, I/D and D/D genotype frequency were 56.5%, 38.9%, 4.6%in controls and 58.0%, 32.9%, 9.0%in T2DM group respectively (P<0.05). The risk in D/D genotype people was 1.916 times than I/I genotype (adjusted OR=1.916, 95%CI=1.199~3.054, P<0.05). The fasting blood sugar of D/D genotype people was significantly higher than that of I/D and I/I genotype people (P<0.05). Conclusions CASP 8 gene-652 6N I/D polymorphisms are associated with T2DM outbreak.