CAJ | 학술논문

目的：研究盐酸椒苯酮胺（PPTA）对庆大霉素（GM）致耳蜗传入神经损伤的保护作用。方法：36只豚鼠随机分3组。正常组：等量生理盐水肌肉注射；GM组：肌肉注射GM 100 mg/（kg·d），14 d；PPTA组：GM致聋并腹腔注射PPTA，10 mg/（kg·d），14 d。听性脑干反应分析听力变化，Western blot 检测耳蜗Caspase-3蛋白表达，TUNEL染色后计算细胞凋亡指数，透射电镜观察形态学改变。结果：实验结束后正常组、GM组和PPTA 组阈值分别为14．58±1．16、65．95±1．17、36．13±1．17；凋亡指数：1．09±0．14、23．17±0．88、8．84±0．49；Caspase-3蛋白：1．09±0．11、2．55±0．20、1．67±0．07，以上指标各组间差异均有显著性（P＜0．05）。形态学观察：GM组耳蜗存在大量凋亡细胞，突触结构破坏严重；PPTA组损伤较轻。结论：PPTA对GM致豚鼠耳蜗传入神经损伤有保护作用。
목적：연구염산초분동알（PPTA）대경대매소（GM）치이와전입신경손상적보호작용。방법：36지돈서수궤분3조。정상조：등량생리염수기육주사；GM조：기육주사GM 100 mg/（kg·d），14 d；PPTA조：GM치롱병복강주사PPTA，10 mg/（kg·d），14 d。은성뇌간반응분석은력변화，Western blot 검측이와Caspase-3단백표체，TUNEL염색후계산세포조망지수，투사전경관찰형태학개변。결과：실험결속후정상조、GM조화PPTA 조역치분별위14．58±1．16、65．95±1．17、36．13±1．17；조망지수：1．09±0．14、23．17±0．88、8．84±0．49；Caspase-3단백：1．09±0．11、2．55±0．20、1．67±0．07，이상지표각조간차이균유현저성（P＜0．05）。형태학관찰：GM조이와존재대량조망세포，돌촉결구파배엄중；PPTA조손상교경。결론：PPTA대GM치돈서이와전입신경손상유보호작용。
Objective To study the protective effect of peperphentonamine hydrochloride (PPTA) on the cochlea afferent nerve system damage induced by gentamicin (GM) of guinea pigs. Methods Thirty-six health guinea pigs were randomly divided into three groups:the normal group with saline; GM group with muscle injection of GM [100 mg/(kg·d),14 d] and PPTA group with peritoneal injection of PPTA [10 mg/(kg·d),14 d] on the ABR was analyzed , and the expression of Caspase-3 was tested by Western blot. TUNEL (after coloration)was performed to observe the apoptotic index,and its morphological changes were observed by TEM. Results After trial, the threshold of control group, GM group, PPTA group were 14.58 ± 1.16, 65.95 ± 1.17, 36.13 ± 1.17;the apoptosis index: 1.09 ± 0.14, 23.17 ± 0.88, 8.84 ± 0.49; the Caspase-3:1.09 ± 0.11, 2.55 ± 0.20, 1.67 ± 0.07. There were significantly difference among three groups (P<0.05). Morphological changes: cells apoptosis and the synapase were damaged seriously in GM group; the injury was less serious in PPTA group. Conclusion PPTA plays a protective role on GM-induced cochlea afferent nerve system damage of guinea pigs.