检验医学与临床
檢驗醫學與臨床
검험의학여림상
JOURNAL OF LABORATORY MEDICINE AND CLINICAL SCIENCES
2014年
20期
2854-2855,2857
,共3页
吴艾霖%熊怡淞%王艳艳%曲远青%段小刚%吴丽娟
吳艾霖%熊怡淞%王豔豔%麯遠青%段小剛%吳麗娟
오애림%웅이송%왕염염%곡원청%단소강%오려연
e 抗原阴性%乙型肝炎病毒%YMDD 突变%HBV 基因型
e 抗原陰性%乙型肝炎病毒%YMDD 突變%HBV 基因型
e 항원음성%을형간염병독%YMDD 돌변%HBV 기인형
e anti-negative%hepatitis B virus%YMDD mutation%HBV genotype
目的:研究感染乙型肝炎病毒患者的乙型肝炎病毒 e 抗原(HBeAg)转阴与长期应用拉米夫定诱发的YMDD 变异和病毒基因型的相关性。方法选取本院确诊的慢性乙型肝炎患者1200例,其中 HBeAg 血清学转阴患者577例,未转阴患者623例,检测患者 HBeAg 、乙型肝炎病毒脱氧核糖核酸(HBV-DNA )和 HBV 基因型,并比较和探讨3者的关系。结果577例 HBeAg 阴性患者中有233例(40.38%)存在病毒复制(HBV-DNA ≥103);对233例基因分型显示,A 、B 、C 、D 基因型分别为163例(69.96%)、57例(24.46%)、11例(4.72%)、2例(0.86%);YMDD 突变检测发现,233例患者中106例(45.49%)发生 YMDD 突变,其中Ⅴ突变68.87%,Ⅰ突变22.64%,Ⅰ/Ⅴ共生突变8.49%。在106例 YMDD 突变患者中 A 、B 、C 、D 基因型分别为0例(0.00%)、72例(67.92%),27例(25.47%),7例(6.60%)。结论本院所在地区 HBeAg 血清转阴的慢性乙型肝炎患者病毒复制仍保持一定的活力,持续监测病毒拷贝数、YMDD 突变和基因型对于此类患者治疗非常必要。
目的:研究感染乙型肝炎病毒患者的乙型肝炎病毒 e 抗原(HBeAg)轉陰與長期應用拉米伕定誘髮的YMDD 變異和病毒基因型的相關性。方法選取本院確診的慢性乙型肝炎患者1200例,其中 HBeAg 血清學轉陰患者577例,未轉陰患者623例,檢測患者 HBeAg 、乙型肝炎病毒脫氧覈糖覈痠(HBV-DNA )和 HBV 基因型,併比較和探討3者的關繫。結果577例 HBeAg 陰性患者中有233例(40.38%)存在病毒複製(HBV-DNA ≥103);對233例基因分型顯示,A 、B 、C 、D 基因型分彆為163例(69.96%)、57例(24.46%)、11例(4.72%)、2例(0.86%);YMDD 突變檢測髮現,233例患者中106例(45.49%)髮生 YMDD 突變,其中Ⅴ突變68.87%,Ⅰ突變22.64%,Ⅰ/Ⅴ共生突變8.49%。在106例 YMDD 突變患者中 A 、B 、C 、D 基因型分彆為0例(0.00%)、72例(67.92%),27例(25.47%),7例(6.60%)。結論本院所在地區 HBeAg 血清轉陰的慢性乙型肝炎患者病毒複製仍保持一定的活力,持續鑑測病毒拷貝數、YMDD 突變和基因型對于此類患者治療非常必要。
목적:연구감염을형간염병독환자적을형간염병독 e 항원(HBeAg)전음여장기응용랍미부정유발적YMDD 변이화병독기인형적상관성。방법선취본원학진적만성을형간염환자1200례,기중 HBeAg 혈청학전음환자577례,미전음환자623례,검측환자 HBeAg 、을형간염병독탈양핵당핵산(HBV-DNA )화 HBV 기인형,병비교화탐토3자적관계。결과577례 HBeAg 음성환자중유233례(40.38%)존재병독복제(HBV-DNA ≥103);대233례기인분형현시,A 、B 、C 、D 기인형분별위163례(69.96%)、57례(24.46%)、11례(4.72%)、2례(0.86%);YMDD 돌변검측발현,233례환자중106례(45.49%)발생 YMDD 돌변,기중Ⅴ돌변68.87%,Ⅰ돌변22.64%,Ⅰ/Ⅴ공생돌변8.49%。재106례 YMDD 돌변환자중 A 、B 、C 、D 기인형분별위0례(0.00%)、72례(67.92%),27례(25.47%),7례(6.60%)。결론본원소재지구 HBeAg 혈청전음적만성을형간염환자병독복제잉보지일정적활력,지속감측병독고패수、YMDD 돌변화기인형대우차류환자치료비상필요。
Objective To study the correlation between hepatitis B e antigen (HBeAg)-negative and HBV gen-otype ,YMDD mutation induced by long-term use of lamivudine in patients with hepatitis B virus infection .Methods 1 200 patients with chronic hepatitis B were recruited in this study ,of which 577 patients were HBeAg negative and 623 were HBeAg positive .Enzyme-linked immunoadsordent assay(ELISA) ,Taqman QPCR and linear probe hybrid-ization were used to detect HBeAg 、HBV-DNA and HBV genotypes respectively .And the relationships between them were analyzed .Results There were 233 patients(40 .38% )with HBV-DNA virus replication (HBV-DNA ≥ 103 ) in all the 577 HBeAg-negetive patients .Further genotyping results showed that there were 163 (69 .96% ) ,57 (24 .46% ) ,11(4 .72% ) and 2(0 .86% ) cases in genotype A ,B ,C and D respectively .YMDD mutation test found that there was 106 cases in 233 patients(45 .49% )with YMDD mutation ,composed by Ⅴ mutation(68 .87% ) ,Ⅰ muta-tions(22 .64% ) ,and Ⅰ/Ⅴ symbiotic mutation(8.49% ) .In the 106 YMDD mutation cases there were 0(0 .00% ) ,72 (67 .92% ) ,27(25 .47% ) ,and 7(6 .60% )cases for genotype A ,B ,C and D respectively .Conclusion Replication of hepatitis B virus was somewhat exist in patients with HBeAg-negative .Continuous monitoring of HBV viruses copy number ,YMDD mutation and genotype is necessary for the treatment of these patients .