CAJ | 학술논문

目的：分析Ⅰ型胶原酶和胰酶在晶状体上皮细胞体外培养中的不同消化作用，以寻找更好的实验方法。方法采用胰酶消化培养法，Ⅰ型胶原酶消化培养法，分别进行晶状体上皮细胞的原代培养，设立对照组和实验组，倒置相差显微镜观察细胞的生长情况，并通过台盼蓝染色观察经消化酶解后游离细胞的存活数量，判别两组间消化作用的差异。结果Ⅰ型胶原酶消化的组织片更容易贴壁，生长速度更快，其游离细胞台盼蓝不着色率为（78．85±6．78）％，胰酶消化法为（27．20±3．28）％，两种酶消化后细胞存活率比较，差异有统计学意义（P＜0．05）。结论Ⅰ型胶原酶消化法培养晶状体上皮细胞的成功率远优于传统改良的胰酶消化法。
목적：분석Ⅰ형효원매화이매재정상체상피세포체외배양중적불동소화작용，이심조경호적실험방법。방법채용이매소화배양법，Ⅰ형효원매소화배양법，분별진행정상체상피세포적원대배양，설립대조조화실험조，도치상차현미경관찰세포적생장정황，병통과태반람염색관찰경소화매해후유리세포적존활수량，판별량조간소화작용적차이。결과Ⅰ형효원매소화적조직편경용역첩벽，생장속도경쾌，기유리세포태반람불착색솔위（78．85±6．78）％，이매소화법위（27．20±3．28）％，량충매소화후세포존활솔비교，차이유통계학의의（P＜0．05）。결론Ⅰ형효원매소화법배양정상체상피세포적성공솔원우우전통개량적이매소화법。
Objective To study the different digestion of type I collagenase and pancreatin cas on epithelial cell of rabbit lens .Methods The anterior capsule obtained from the mature rabbit ,and then isolated and cultured the ep-ithelial cell of rabbit lens in vitro by type I collagenase and pancreatin cas respectively .Experimental group and con-trol group were established ,the growth of the cells was observed with inverted microscope ,and the survival rate of the cells was determined by trypan blue staining method .Results The growth of lens epithelial cell in experimental group was faster ,the survival rate of the cells in experimental group was (78 .85 ± 6 .78)% ,and that of control group was (27 .20 ± 3 .28)% .There was statistically significant difference between the two groups (P< 0 .05) .Conclusion The survival rate of rabbit lens epithelial cell cultured by type I collagenase in Vitro is far better than that by the tra -ditional method using pancreatin cas .