中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
12期
1070-1072
,共3页
白银亮%徐伟%唐慧如%李静%颜抒阳%连佛颜
白銀亮%徐偉%唐慧如%李靜%顏抒暘%連彿顏
백은량%서위%당혜여%리정%안서양%련불안
黄芩苷%吗啡%行为敏化%多巴胺
黃芩苷%嗎啡%行為敏化%多巴胺
황금감%마배%행위민화%다파알
Baicalin%Morphine%Behavioral sensitization%Dopamine
目的 探讨黄芩苷对小鼠吗啡行为敏化的影响.方法 测定小鼠的自主活动,观察黄芩苷对小鼠自主活动的影响.分别采用单次给予吗啡(10 mg·kg-1,ip)致小鼠高活动性及反复间断给予吗啡处理建立小鼠吗啡行为敏化模型,观察黄芩苷对吗啡致高活动性及行为敏化形成和表达的影响.酶联免疫吸附法检测小鼠大脑中脑腹侧背盖区(VTA)和前额叶(PFC)区多巴胺含量变化.结果 黄芩苷(30,60,120 mg·kg-1,ig)显著抑制小鼠自主活动[对照(1095.8± 174.5)次,黄芩苷(899.6± 187.2)次、(724.2±221.4)次、(609.1± 154.6)次;P＜0.01]和急性吗啡诱导的小鼠高活动性[模型组(1518.2±185.8)次,黄芩苷(1385.4± 224.2)次、(1205.1±174.6)次、(1100.3±235.1)次;P＜0.01];黄芩苷呈剂量依赖地阻断小鼠吗啡行为敏化的形成和表达[(模型组(2096.2±304.6)次,黄芩苷(2004.2±218.5)次、(1998.7±224.3)次、(1836.1±233.5)次;P＜0.05);(模型组(2124.2± 189.6)次,黄芩苷(1922.2±314.7)次、(1524.1±289.2)次、(1477.4± 219.3)次;P＜0.01)].黄芩苷抑制吗啡敏化小鼠大脑VTA和PFC区多巴胺的释放[模型组(457.6±92.1)μg·L-1,(589.2±102.5) μg·L-1,黄芩苷(391.1±56.8) μg·L1,(448.6±99.3) μg·L-1;(324.5±66.2) μg·L1,(368.7±45.9) μg·L-1;(234.3±52.6) μg·L-1,(305.3±84.1) μg·L-1;P＜0.01,P＜0.01].结论 黄芩苷对小鼠吗啡行为敏化的形成和表达具有抑制作用,这种作用与其抑制大脑VTA和PFC区多巴胺的过度释放有关.
目的 探討黃芩苷對小鼠嗎啡行為敏化的影響.方法 測定小鼠的自主活動,觀察黃芩苷對小鼠自主活動的影響.分彆採用單次給予嗎啡(10 mg·kg-1,ip)緻小鼠高活動性及反複間斷給予嗎啡處理建立小鼠嗎啡行為敏化模型,觀察黃芩苷對嗎啡緻高活動性及行為敏化形成和錶達的影響.酶聯免疫吸附法檢測小鼠大腦中腦腹側揹蓋區(VTA)和前額葉(PFC)區多巴胺含量變化.結果 黃芩苷(30,60,120 mg·kg-1,ig)顯著抑製小鼠自主活動[對照(1095.8± 174.5)次,黃芩苷(899.6± 187.2)次、(724.2±221.4)次、(609.1± 154.6)次;P＜0.01]和急性嗎啡誘導的小鼠高活動性[模型組(1518.2±185.8)次,黃芩苷(1385.4± 224.2)次、(1205.1±174.6)次、(1100.3±235.1)次;P＜0.01];黃芩苷呈劑量依賴地阻斷小鼠嗎啡行為敏化的形成和錶達[(模型組(2096.2±304.6)次,黃芩苷(2004.2±218.5)次、(1998.7±224.3)次、(1836.1±233.5)次;P＜0.05);(模型組(2124.2± 189.6)次,黃芩苷(1922.2±314.7)次、(1524.1±289.2)次、(1477.4± 219.3)次;P＜0.01)].黃芩苷抑製嗎啡敏化小鼠大腦VTA和PFC區多巴胺的釋放[模型組(457.6±92.1)μg·L-1,(589.2±102.5) μg·L-1,黃芩苷(391.1±56.8) μg·L1,(448.6±99.3) μg·L-1;(324.5±66.2) μg·L1,(368.7±45.9) μg·L-1;(234.3±52.6) μg·L-1,(305.3±84.1) μg·L-1;P＜0.01,P＜0.01].結論 黃芩苷對小鼠嗎啡行為敏化的形成和錶達具有抑製作用,這種作用與其抑製大腦VTA和PFC區多巴胺的過度釋放有關.
목적 탐토황금감대소서마배행위민화적영향.방법 측정소서적자주활동,관찰황금감대소서자주활동적영향.분별채용단차급여마배(10 mg·kg-1,ip)치소서고활동성급반복간단급여마배처리건립소서마배행위민화모형,관찰황금감대마배치고활동성급행위민화형성화표체적영향.매련면역흡부법검측소서대뇌중뇌복측배개구(VTA)화전액협(PFC)구다파알함량변화.결과 황금감(30,60,120 mg·kg-1,ig)현저억제소서자주활동[대조(1095.8± 174.5)차,황금감(899.6± 187.2)차、(724.2±221.4)차、(609.1± 154.6)차;P＜0.01]화급성마배유도적소서고활동성[모형조(1518.2±185.8)차,황금감(1385.4± 224.2)차、(1205.1±174.6)차、(1100.3±235.1)차;P＜0.01];황금감정제량의뢰지조단소서마배행위민화적형성화표체[(모형조(2096.2±304.6)차,황금감(2004.2±218.5)차、(1998.7±224.3)차、(1836.1±233.5)차;P＜0.05);(모형조(2124.2± 189.6)차,황금감(1922.2±314.7)차、(1524.1±289.2)차、(1477.4± 219.3)차;P＜0.01)].황금감억제마배민화소서대뇌VTA화PFC구다파알적석방[모형조(457.6±92.1)μg·L-1,(589.2±102.5) μg·L-1,황금감(391.1±56.8) μg·L1,(448.6±99.3) μg·L-1;(324.5±66.2) μg·L1,(368.7±45.9) μg·L-1;(234.3±52.6) μg·L-1,(305.3±84.1) μg·L-1;P＜0.01,P＜0.01].결론 황금감대소서마배행위민화적형성화표체구유억제작용,저충작용여기억제대뇌VTA화PFC구다파알적과도석방유관.
Objective To investigate the effects of baicalin on morphine-induced behavioral sensitization.Methods Locomotor activity was measured for 2h after administration with baicalin in mice.Hyperlocomotion induced by acute morphine (10 mg· kg-1,ip) and behavioral sensitization induced by repeated morphine were established.The level of dopamine of ventral tegmental area(VTA) and prefrontal cortex(PFC) in mice was tested by ELISA assay.Results Baiealin inhibited significantly both locomotor activity in mice (control (1095.8 ± 174.5) times,baicalin (899.6± 187.2),(724.2± 221.4),(609.1 ± 154.6) times ; P＜ 0.01) and hyperlocomotion induced by acute morphine(model (1518.2± 185.8) times,baicalin (1385.4±224.2),(1205.1 ± 174.6),(1100.3±235.1) times ; P＜0.01).Similar inhibition was also seen in the development and expression of morphine-induced behavioral sensitization(model(2096.2±304.6) times,baicalin (2004.2 ± 218.5),(1998.7-± 224.3),(1836.1 ± 233.5) times,P＜ 0.05 ; model (2124.2 ± 189.6) times,baicalin (1922.2± 314.7),(1524.1±289.2),(1477.4± 219.3) times,P＜0.01).Baicalin inhibited dopamine release in VTA and PFC of morphine-sensitized mice(model(457.6± 92.1,589.2 ±102.5) μg · L-1,baicalin(391.1±56.8) μg · L-1,(448.6± 99.3) μg · L-1; (324.5±66.2) μg · L-1,(368.7±45.9) μg · L 1 ; (234.3± 52.6) μg · L-1,(305.3±84.1) μg · L-1 ; P＜0.01,P＜0.01).Conclusion Baicalin inhibits the development and the expression of morphine-induced behavioral sensitization in mice,and this effect is related to the inhibition of dopamine release in VTA and PFC of mice.
