中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
5期
436-439
,共4页
赵林%张梁%孙欣羊%宋红涛%范惠民%张理义%钟爱芳%师征
趙林%張樑%孫訢羊%宋紅濤%範惠民%張理義%鐘愛芳%師徵
조림%장량%손흔양%송홍도%범혜민%장리의%종애방%사정
小分子RNA%非特异性精神发育迟滞%基因芯片
小分子RNA%非特異性精神髮育遲滯%基因芯片
소분자RNA%비특이성정신발육지체%기인심편
miRNAs%Non-specific mental retardation%Gene chips
目的 研究非特异性精神发育迟滞患者外周血中miRNA的异常表达.方法 采用基因芯片筛查技术对3例非特异性精神发育迟滞儿童和健康儿童外周静脉血的miRNA进行筛查,进行生物信息学分析,并选取其中10个感兴趣miRNA,运用实时定量PCR技术在34例非特异性精神发育迟滞患者与32例正常个体之间进一步验证芯片筛查的结果.结果 经筛查27个miRNA的表达差异具有统计学意义(上调或者下调倍数变化值≥2.0,P≤0.05),其中22个表达上调,5个表达下调.经实时定量PCR验证miR-664a-5p,miR-1207-5p,miR-3141,miR-1228-5p,miR-4505在病例组(3.93±3.82,2.48±3.45,2.75±3.62,10.03±3.59,5.35±3.44)中表达较对照组(5.47±3.59,3.95±1.3.57,4.14±3.68,13.23±5.05,6.85±3.22)上调,差异有统计学意义(P＜0.05 ～ 0.01).结论 多种miRNA在非特异性精神发育迟滞患者外周血中有异常表达.
目的 研究非特異性精神髮育遲滯患者外週血中miRNA的異常錶達.方法 採用基因芯片篩查技術對3例非特異性精神髮育遲滯兒童和健康兒童外週靜脈血的miRNA進行篩查,進行生物信息學分析,併選取其中10箇感興趣miRNA,運用實時定量PCR技術在34例非特異性精神髮育遲滯患者與32例正常箇體之間進一步驗證芯片篩查的結果.結果 經篩查27箇miRNA的錶達差異具有統計學意義(上調或者下調倍數變化值≥2.0,P≤0.05),其中22箇錶達上調,5箇錶達下調.經實時定量PCR驗證miR-664a-5p,miR-1207-5p,miR-3141,miR-1228-5p,miR-4505在病例組(3.93±3.82,2.48±3.45,2.75±3.62,10.03±3.59,5.35±3.44)中錶達較對照組(5.47±3.59,3.95±1.3.57,4.14±3.68,13.23±5.05,6.85±3.22)上調,差異有統計學意義(P＜0.05 ～ 0.01).結論 多種miRNA在非特異性精神髮育遲滯患者外週血中有異常錶達.
목적 연구비특이성정신발육지체환자외주혈중miRNA적이상표체.방법 채용기인심편사사기술대3례비특이성정신발육지체인동화건강인동외주정맥혈적miRNA진행사사,진행생물신식학분석,병선취기중10개감흥취miRNA,운용실시정량PCR기술재34례비특이성정신발육지체환자여32례정상개체지간진일보험증심편사사적결과.결과 경사사27개miRNA적표체차이구유통계학의의(상조혹자하조배수변화치≥2.0,P≤0.05),기중22개표체상조,5개표체하조.경실시정량PCR험증miR-664a-5p,miR-1207-5p,miR-3141,miR-1228-5p,miR-4505재병례조(3.93±3.82,2.48±3.45,2.75±3.62,10.03±3.59,5.35±3.44)중표체교대조조(5.47±3.59,3.95±1.3.57,4.14±3.68,13.23±5.05,6.85±3.22)상조,차이유통계학의의(P＜0.05 ～ 0.01).결론 다충miRNA재비특이성정신발육지체환자외주혈중유이상표체.
Objective To explore the differentially expressed miRNAs in peripheral blood firom the patients with non-specific mental retardation.Methods The miRNAs expression profilings in peripheral blood of 3 pairs in non-specific mental retardation patients and normal control were analyzed using microRNA array.10 miRNAs were selected according to the microRNA array results.The expression of these miRNAs were detected by quantitative RT-PCR in 34 non-specific mental retardation patients and 32 healthy individuals.Results There were 27 kinds of microRNA which had statistically different expression(FC ≥ 2.0,P≤ 0.05),and among the above abnormal expressed miRNAs,22 were up-expressed (over-expression),5 were down-expressed (under-expression).The expression levels of miR-664a-5p,miR-1207-5p,miR-3141,miR-1228-5p and miR-4505 in non-specific mental retardation patients group (3.93 ± 3.82,2.48± 3.45,2.75 ± 3.62,10.03 ± 3.59,5.35 ± 3.44,respectively) were significantly up-expressed compared with the control group (5.47 ± 3.59,3.95 ± 1.3.57,4.14 ± 3.68,13.23 ± 5.05,6.85±3.22,P＜0.05-0.01).Conclusion There are many differentially expressed miRNAs between the patients with non-specific mental retardation and healthy controls.
