CAJ | 학술논문

目的初步探讨信号转导与转录活化因子3(Stat3)与绒癌细胞侵袭转移能力的关系.方法体外培养人绒毛膜癌细胞系JAR(低侵袭),JEG-3(高侵袭)及收集人正常早孕绒毛组织;运用Transwell体外侵袭实验检测并比较JAR和JEG-3细胞的侵袭转移潜能;采用Western-blot检测JAR、JEG-3细胞以及人正常早孕绒毛组织stat3及其活性形式P-star3蛋白的表达情况;同时用免疫组织细胞化学检测JAR和JEG-3细胞stat3及postat3蛋白的表达情况.结果在人绒毛膜癌细胞中检测到stat3,p-star3的高表达;JEG-3细胞侵袭至基质胶膜背面的数量较JAR细胞明显增多(P<0.05);stat3及p-star3蛋白在绒癌细胞的表达强于正常绒毛组织(P<0.01),并且JEG-3细胞较JAR细胞显著增强(P<0.05).结论 stat3及p-star3在绒癌细胞中较正常绒毛组织高表达,且在JEG-3细胞的表达高于JAR细胞.
목적 초보탐토신호전도여전록활화인자3(Stat3)여융암세포침습전이능력적관계.방법 체외배양인융모막암세포계JAR(저침습),JEG-3(고침습)급수집인정상조잉융모조직;운용Transwell체외침습실험검측병비교JAR화JEG-3세포적침습전이잠능;채용Western-blot검측JAR、JEG-3세포이급인정상조잉융모조직stat3급기활성형식P-star3단백적표체정황;동시용면역조직세포화학검측JAR화JEG-3세포stat3급postat3단백적표체정황.결과 재인융모막암세포중검측도stat3,p-star3적고표체;JEG-3세포침습지기질효막배면적수량교JAR세포명현증다(P<0.05);stat3급p-star3단백재융암세포적표체강우정상융모조직(P<0.01),병차JEG-3세포교JAR세포현저증강(P<0.05).결론 stat3급p-star3재융암세포중교정상융모조직고표체,차재JEG-3세포적표체고우JAR세포.
Objective To explore the relationship between the expression of signal transducers and activators of transcription 3 (stat3),the invasiveness and metastasis mechanism of choriocarcinoma cells.Method Choriocarcinoma cell lines(JEG-3 cells and JAR cell)were cultured in vitro,and the human chorionic villi tissues were collected.The invasiveness was assessed by penetration through a matrigel-coated filter in transwell chamber ability between JEG-3 cells and JAR cell.Expression of star3 and p-star3 protein in the human chorionic villi tissues and choriocarcinoma cell lines(JEG-3 cells and JAR ceils)were detected by western blot and immunocytochemistry.Result The expression of stat3 and p-stat3 protein can be found in choriocarcinoma cell lines.The amount of JEG-3 cells penetrating through matrigel-coated filter in transweil chamber was significantly increased,compared with JAR cells(P<0.05).Protein expression level of star3 and p-star3 was significantly increased in JEG-3 cell line,compared with JAR cell line,and it was markedly increased in choriocarcinoma cell lines compared with normal villi tissues(P<0.01).SP immunocytocbemical results also showed that the expression of star3 and p-star3 was significantly increased in JEG-3 cell line,compared with JAR cell line(P<0.05).Conclusions The expression of stat3 and p-star3 protein in choriocarcinonm cells Was higher than that in normal villi tissues,and which in JEG-3 cells Was higher than that in JAR cells.