CAJ | 학술논문

目的通过观察L-精氨酸对胎儿生长受限胎盘Bcl-2、Bax凋亡蛋白表达的影响,探讨L-精氨酸对FGR治疗效果及作用机制.方法选择FGR患者60例,随机分为A组(常规治疗组)30例,B组(L-精氨酸治疗组)30例,生后测量新生儿体重,分娩后10 min内取胎盘中央组织,10%甲醛固定后石蜡包埋,免疫组化法观察胎盘Bel-2、Bax凋亡蛋白的表达情况.结果 A组较B组Bax表达量相对增强而bcl-2表达相对减弱.结论 L-精氨酸可以降低胎盘细胞凋亡相关基因Bax蛋白的表达,而增加Bel-2的表达,从而减少胎盘细胞凋亡,改善胎盘功能,促进胎儿发育.
목적 통과관찰L-정안산대태인생장수한태반Bcl-2、Bax조망단백표체적영향,탐토L-정안산대FGR치료효과급작용궤제.방법 선택FGR환자60례,수궤분위A조(상규치료조)30례,B조(L-정안산치료조)30례,생후측량신생인체중,분면후10 min내취태반중앙조직,10%갑철고정후석사포매,면역조화법관찰태반Bel-2、Bax조망단백적표체정황.결과 A조교B조Bax표체량상대증강이bcl-2표체상대감약.결론 L-정안산가이강저태반세포조망상관기인Bax단백적표체,이증가Bel-2적표체,종이감소태반세포조망,개선태반공능,촉진태인발육.
Objectives To study the effect and mechanism of L-arginine to Fetal Growth Restriction(FGR)by observing the expression of bcl-2 and bax in placenta.Methods Sixty cages were enrolled in this study,among which,30 cases treated with conventional ways was named group A.and the other 30 cases treated with L-arginine was named group B.The birth weight was compared.Then the central tissue of placenta got within 10 min after delivery were fixed by 10 percent formaldehyde and embed by paraffin wax to observe the expression of bcl-2 and bax using immunohistochemistry.Results Compared with group A,the healing rate and birth weight of group B is higher(P<0.05).The expression of bax in group A increased while bcl-2 decreased,compared with group B.Conclusiom L-arginine could degrade the expression of bax protein and promote the expression of bcl-2,which could contribute to decrease apoptosis,improve the function of pLacenta and increase the development of fetal.