中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
10期
1317-1320
,共4页
旷文勇%周新伏%李睿娟%郑敏翠%张广森
曠文勇%週新伏%李睿娟%鄭敏翠%張廣森
광문용%주신복%리예연%정민취%장엄삼
胎血%细胞培养技术/方法%造血干细胞%骨髓细胞
胎血%細胞培養技術/方法%造血榦細胞%骨髓細胞
태혈%세포배양기술/방법%조혈간세포%골수세포
Fetal blood%Cell culture techniques/MT%Hematopoietic stem cells%Bone marrow cells
目的 观察脐带血清体外培养人骨髓间充质干细胞的生物学特性.方法 抽取骨髓穿刺液10 ml,采用含10%脐带血清的DMEM/F12培养,流式细胞仪检测细胞表面抗原,成骨诱导体系及脂肪诱导体系诱导BM-MSCs定向分化.结果 BM-MSCSs高表达CD29、CD73、CD105,不表达CD34、CD45、CD31,BM-MSCs体外能诱导为成骨细胞和脂肪细胞.结论 脐带血清培养的BM-MSCs的具有较高的纯度和体外分化能力,脐带血清培养BM-MSCs适合临床应用.
目的 觀察臍帶血清體外培養人骨髓間充質榦細胞的生物學特性.方法 抽取骨髓穿刺液10 ml,採用含10%臍帶血清的DMEM/F12培養,流式細胞儀檢測細胞錶麵抗原,成骨誘導體繫及脂肪誘導體繫誘導BM-MSCs定嚮分化.結果 BM-MSCSs高錶達CD29、CD73、CD105,不錶達CD34、CD45、CD31,BM-MSCs體外能誘導為成骨細胞和脂肪細胞.結論 臍帶血清培養的BM-MSCs的具有較高的純度和體外分化能力,臍帶血清培養BM-MSCs適閤臨床應用.
목적 관찰제대혈청체외배양인골수간충질간세포적생물학특성.방법 추취골수천자액10 ml,채용함10%제대혈청적DMEM/F12배양,류식세포의검측세포표면항원,성골유도체계급지방유도체계유도BM-MSCs정향분화.결과 BM-MSCSs고표체CD29、CD73、CD105,불표체CD34、CD45、CD31,BM-MSCs체외능유도위성골세포화지방세포.결론 제대혈청배양적BM-MSCs적구유교고적순도화체외분화능력,제대혈청배양BM-MSCs괄합림상응용.
Objective To observe biological characteristics of adult human marrow mesenchymal stem cells(BM-MSCs)cultured by the umbilical cord serum.Methods BM-MSCs were obtained from fresh adult human bone marrow and cultured with DMEM/F12 which included 10%umbilical cord blood serum.The surface antigens of BM-MSCs were detected by flow cytometry.BM-MSCs were cultured into differentiation of osteoblasts and adipocytes with conditioned medium for osteogenesis and adipogenesis. Results The antigen of CD29.CD73,and CD105 were high expressed on BM-MSCs.The antigen of CD34,CD45 and CD31 were not expressed on BM-MSCs.The BMMSCs were differentiated into osteoblasts and adipocytes.Conclusion BM-MSCs have high purity and differentiation potency under cuhuring with umbilical cord blood serum.The umbilical cord serum is adapted to clinical application.
