CAJ | 학술논문

本试验旨在研究天蚕素A-马盖宁杂合肽对小鼠小肠黏膜结构、黏膜免疫功能和肠道菌群的影响。选取18只健康且体重相近的 BALB/c 小鼠随机分成3组：对照组（生理盐水0．75 mL/d灌胃）、低剂量杂合肽组（0．26 mg/mL的杂合肽0．75 mL/d灌胃）、高剂量杂合肽组（0．52 mg/mL的杂合肽0．75 mL/d灌胃）。试验期为6周。结果表明：1）2个杂合肽组十二指肠绒毛长度均显著大于对照组（ P＜0．05），各段小肠的隐窝深度均显著低于对照组（ P＜0．05），各段小肠绒毛长度/隐窝深度均显著高于对照组（ P＜0．05）。2）与对照组相比，2个杂合肽组各段小肠黏膜内免疫球蛋白A阳性表达水平均显著升高（P＜0．05）。3）2个杂合肽组小肠黏膜内白细胞介素（IL）-2、干扰素-γ（IFN-γ）及IL-4含量均显著高于对照组（P＜0．05），而IFN-γ/IL-4各组之间差异不显著（ P＞0．05）。4）2个杂合肽组盲肠内容物中的大肠杆菌数量均显著低于对照组（P＜0．05），双歧杆菌与乳酸杆菌数量均显著高于对照组（P＜0．05）。由此得出，天蚕素A-马盖宁杂合肽灌胃能改善机体小肠黏膜结构；可促进免疫球蛋白A的表达来提高小肠黏膜免疫防御功能；可促进IL-2及IFN-γ的分泌来提高肠道细胞免疫水平，促进IL-4的分泌以提高肠道体液免疫水平并能够保持辅助性T细胞（ Th）1/Th2的平衡状态；可有效降低肠道致病菌大肠杆菌的数量并显著增加肠道益生菌双歧杆菌和乳酸杆菌的数量。
본시험지재연구천잠소A-마개저잡합태대소서소장점막결구、점막면역공능화장도균군적영향。선취18지건강차체중상근적 BALB/c 소서수궤분성3조：대조조（생리염수0．75 mL/d관위）、저제량잡합태조（0．26 mg/mL적잡합태0．75 mL/d관위）、고제량잡합태조（0．52 mg/mL적잡합태0．75 mL/d관위）。시험기위6주。결과표명：1）2개잡합태조십이지장융모장도균현저대우대조조（ P＜0．05），각단소장적은와심도균현저저우대조조（ P＜0．05），각단소장융모장도/은와심도균현저고우대조조（ P＜0．05）。2）여대조조상비，2개잡합태조각단소장점막내면역구단백A양성표체수평균현저승고（P＜0．05）。3）2개잡합태조소장점막내백세포개소（IL）-2、간우소-γ（IFN-γ）급IL-4함량균현저고우대조조（P＜0．05），이IFN-γ/IL-4각조지간차이불현저（ P＞0．05）。4）2개잡합태조맹장내용물중적대장간균수량균현저저우대조조（P＜0．05），쌍기간균여유산간균수량균현저고우대조조（P＜0．05）。유차득출，천잠소A-마개저잡합태관위능개선궤체소장점막결구；가촉진면역구단백A적표체래제고소장점막면역방어공능；가촉진IL-2급IFN-γ적분비래제고장도세포면역수평，촉진IL-4적분비이제고장도체액면역수평병능구보지보조성T세포（ Th）1/Th2적평형상태；가유효강저장도치병균대장간균적수량병현저증가장도익생균쌍기간균화유산간균적수량。
This experiment was to study the effects of cecropin A-magainin hybrid peptide on small intestinal mucosal structure, mucosal immune function and intestinal microflora in mice. The eighteen healthy BALB/c mice with similar weight were randomly divided into three groups: control group ( given 0.75 mL saline water by gastric lavage) , low dose of hybrid peptide group ( given 0.75 mL 0.26 mg/mL cecA-mag peptide by gas-tric lavage) , and high dose of hybrid peptide group ( given 0.75 mL 0.52 mg/mL cecA-mag peptide by gastric lavage). The feeding experiment lasted for 6 weeks. The results showed as follows: 1) the villous length of duodenum in two cecA-mag peptide groups was higher than that in control group ( P<0.05);the crypt depth of every part of small intestinal in two cecA-mag peptide groups was significantly lower than that in control group (P<0.05); and villous length/crypt depth (V/C) of every part of small intestinal was significantly higher than that in control group ( P<0.05) . 2) Compared with control group, the positive expression levels of IgA in duodenum, jejunum, and ileum were significantly higher in two cecA-mag peptide groups ( P<0.05) . 3) The contents of IL-2, IFN-γ and IL-4 in two cecA-mag peptide groups were significantly higher than those in con-trol group (P<0.05). IFN-γ/IL-4 was not significantly different among different groups (P>0.05). 4) The number of Escherichia coli in two cecA-mag peptide groups was significantly lower than that in control group ( P<0.05) , the number of Bifidobacterium and Lactobacillus in two cecA-mag peptide groups was significantly higher than that in control group (P<0.05). It is concluded that cecropin A-magainin hybrid peptide can im-prove mucosal structure of small intestine and may enhance intestinal mucosal immune function through enhan-cing the expression of IgA, improve cellular immunity through enhancing the secreting of IL-2 and IFN-γ, im-prove humoral immunity through enhancing the secreting of IL-4, keep the balance of Th1/Th2, effectsively decrease the number of Escherichia coli of pathogenic bacteria and increase the number of Bifidobacterium and Lactobacillus of intestinal probiotics.