CAJ | 학술논문

本文旨在研究饲料中添加甘露寡糖对团头鲂幼鱼生长性能、抗氧化能力和肠道菌群的影响。选用平均体重为（1．05±0．01） g的团头鲂幼鱼360尾，随机分为4组（每组3个重复，每个重复30尾），分别饲喂添加0（对照组）、100、200和400 mg/kg甘露寡糖的试验饲料，试验期为8周。结果表明：饲料中添加甘露寡糖对团头鲂幼鱼的增重率、饲料系数和成活率均无显著影响（ P＞0．05）。与对照组相比，饲料中添加100、200和400 mg/kg甘露寡糖显著提高了肝脏超氧化物歧化酶和过氧化氢酶活性（P＜0．05），显著降低了肝脏丙二醛含量（P＜0．05）。变性梯度凝胶电泳（ DGGE）指纹图谱结果分析表明，200和400 mg/kg甘露寡糖组团头鲂幼鱼肠道菌群Shannon-Wiener指数显著低于对照组（P＜0．05），其优势菌主要为放线菌门（Actinobacteria）、变形菌门（ Proteobacteria）和硬壁菌门（ Firmicutes）。由此可见，饲料中添加200～400 mg/kg甘露寡糖能提高团头鲂幼鱼的抗氧化能力，改善肠道菌群结构。
본문지재연구사료중첨가감로과당대단두방유어생장성능、항양화능력화장도균군적영향。선용평균체중위（1．05±0．01） g적단두방유어360미，수궤분위4조（매조3개중복，매개중복30미），분별사위첨가0（대조조）、100、200화400 mg/kg감로과당적시험사료，시험기위8주。결과표명：사료중첨가감로과당대단두방유어적증중솔、사료계수화성활솔균무현저영향（ P＞0．05）。여대조조상비，사료중첨가100、200화400 mg/kg감로과당현저제고료간장초양화물기화매화과양화경매활성（P＜0．05），현저강저료간장병이철함량（P＜0．05）。변성제도응효전영（ DGGE）지문도보결과분석표명，200화400 mg/kg감로과당조단두방유어장도균군Shannon-Wiener지수현저저우대조조（P＜0．05），기우세균주요위방선균문（Actinobacteria）、변형균문（ Proteobacteria）화경벽균문（ Firmicutes）。유차가견，사료중첨가200～400 mg/kg감로과당능제고단두방유어적항양화능력，개선장도균군결구。
This experiment was conducted to investigate the effects of dietary mannose oliogosaccharides ( MOS) on growth performance, antioxidant ability and intestinal microflora of juvenile blunt anout bream ( Megalobrama amblycephala) . A total of 360 juvenile blunt anout bream with the average body weight of (1.05±0.01) g were randomly divided into 4 groups with 3 replicates per group and 30 fish per replicate. The fish in control group were fed a basal diet, and the fish in MOS supplemental groups were fed the experimental diets supplemmented with 100, 200 and 400 mg/kg MOS on the basis of basal diet, respectively. After 8 weeks feeding, the results showed as follows: there were no significantly differences in weight gain rate, feed conversion ratio and survival rate among all groups ( P>0.05) . Compared with control group, supplementation with 100, 200 and 400 mg/kg MOS significantly improved the liver superoxide dismutase and catalase activi-ties ( P<0.05) , while significantly decreased the liver malonaldehyde content ( P<0.05) . Furthermore, the de-natured gradient gel electrophoresis ( DGGE) fingerprints demonstrated that the Shannon-Wiener index of intes-tinal microflora in 200 and 400 mg/kg MOS groups was significantly lower than that in control group ( P<0.05) , and the main predominant bacteria in 200 and 400 mg/kg MOS groups were Actinobacteria, Pro-teobacteria and Firmicutes. It is concluded that supplementation with 200 to 400 mg/kg MOS can elevate the antioxidant ability and improve the intestinal microflora structure of juvenile blunt anout bream.