CAJ | 학술논문

本研究旨在通过研究饲粮铜添加水平对黄羽肉公鸡生长性能、抗氧化性能、肝脏和血清铜含量等的影响，以探讨43～63日龄黄羽肉公鸡铜的需要量。选用43日龄健康、发育良好的快大型岭南黄羽肉公鸡900只，根据体重随机分为6个组，每组6个重复，每个重复25只鸡，组1（对照组）为基础饲粮组（铜水平为2．74 mg/kg），组2～6饲粮在基础饲粮中分别添加4、8、16、32、64 mg/kg铜。结果表明：1）与对照组相比，16和32 mg/kg铜组显著提高试鸡末重、平均日增重和平均日采食量（P＜0．05），饲粮中铜添加水平对料重比无显著影响（P＞0．05）。2）与对照组相比，16和32 mg/kg铜组血清铜锌-超氧化物歧化酶（ CuZn-SOD）活性显著升高（ P＜0．05），32和64 mg/kg铜组肝脏CuZn-SOD活性显著升高（P＜0．05），饲粮铜添加水平对胸肌中 CuZn-SOD活性无显著影响（ P＞0．05）；饲粮添加32和64 mg/kg铜显著降低了胸肌丙二醛（ MDA）含量（P＜0．05），各组间血清和肝脏MDA含量均无显著差异（P＞0．05）；添加16、32、64 mg/kg铜显著降低了血清铜蓝蛋白活性（P＜0．05）。3）与对照组相比，8、16 mg/kg铜组肝脏铜含量显著降低（P＜0．05），铜添加水平对血清铜含量和肝脏金属硫蛋白含量影响不显著（P＞0．05）。综合考虑各项指标，为获得较好生长性能和抗氧化性能，43～63日龄黄羽肉公鸡饲粮铜适宜添加水平分别为16和32 mg/kg，加上基础饲粮铜水平为2．74 mg/kg，则该阶段黄羽肉公鸡铜需要量分别为18．74和34．74 mg/kg；以生长性能和肝脏CuZn-SOD活性为依据，通过折线回归分析估测得到43～63日龄黄羽肉公鸡铜需要量分别为19．94和38．34 mg/kg。
본연구지재통과연구사량동첨가수평대황우육공계생장성능、항양화성능、간장화혈청동함량등적영향，이탐토43～63일령황우육공계동적수요량。선용43일령건강、발육량호적쾌대형령남황우육공계900지，근거체중수궤분위6개조，매조6개중복，매개중복25지계，조1（대조조）위기출사량조（동수평위2．74 mg/kg），조2～6사량재기출사량중분별첨가4、8、16、32、64 mg/kg동。결과표명：1）여대조조상비，16화32 mg/kg동조현저제고시계말중、평균일증중화평균일채식량（P＜0．05），사량중동첨가수평대료중비무현저영향（P＞0．05）。2）여대조조상비，16화32 mg/kg동조혈청동자-초양화물기화매（ CuZn-SOD）활성현저승고（ P＜0．05），32화64 mg/kg동조간장CuZn-SOD활성현저승고（P＜0．05），사량동첨가수평대흉기중 CuZn-SOD활성무현저영향（ P＞0．05）；사량첨가32화64 mg/kg동현저강저료흉기병이철（ MDA）함량（P＜0．05），각조간혈청화간장MDA함량균무현저차이（P＞0．05）；첨가16、32、64 mg/kg동현저강저료혈청동람단백활성（P＜0．05）。3）여대조조상비，8、16 mg/kg동조간장동함량현저강저（P＜0．05），동첨가수평대혈청동함량화간장금속류단백함량영향불현저（P＞0．05）。종합고필각항지표，위획득교호생장성능화항양화성능，43～63일령황우육공계사량동괄의첨가수평분별위16화32 mg/kg，가상기출사량동수평위2．74 mg/kg，칙해계단황우육공계동수요량분별위18．74화34．74 mg/kg；이생장성능화간장CuZn-SOD활성위의거，통과절선회귀분석고측득도43～63일령황우육공계동수요량분별위19．94화38．34 mg/kg。
This experiment was conducted to investigate the effects of dietary copper level on growth perform-ance, antioxidative property, copper content in serum and liver of male yellow-feathered broilers aged from 43 to 63 days, and then to estimate the dietary copper requirement of male yellow-feathered broilers. Nine hundred male fast large Lingnan yellow-feathered broilers aged 43 days with healthy and well developed body were ran-domly assigned to six dietary groups. Each group consisted of six replicates with 25 birds per replicate. Group 1 ( control group) was fed with the basal diet ( copper level was 2.74 mg/kg) , and group 2 to 6 were fed with the basal diet added with 4, 8, 16, 32 and 64 mg/kg copper, respectively. Results showed as follows: 1) compared with control group, the final weight, average daily gain and average daily feed intake of broilers in 16 and 32 mg/kg copper groups were significantly higher ( P<0.05) , but there was no significant difference in the ratio of feed to gain among all groups ( P>0.05) . 2) Compared with control group, the CuZn-superoxide dismutase ( CuZn-SOD) activity in serum in 16 and 32 mg/kg copper groups was significantly higher ( P<0.05) . The CuZn-SOD activity in liver was significantly higher in 32 and 64 mg/kg copper groups ( P<0.05) . There was no significant difference in CuZn-SOD activity of breast muscle (P>0.05).Dietary copper level did not affect the content of malondialdehyde ( MDA) in serum and liver of broilers ( P>0.05) . The MDA content in breast muscle in 32 and 64 mg/kg copper groups was significantly lower ( P<0.05) . The ceruloplasmin ac-tivity in serum in 16, 32 and 64 mg/kg copper groups was significantly lower (P<0.05).3) Compared with control group, the copper content of liver in 8 and 16 mg/kg copper groups was significantly lower ( P<0.05) . But copper addition had no significant effects on the content of copper in serum and the content of metallothio-nein in liver ( P>0.05) . In conclusion, based on the growth performance and antioxidative property, the apro-priate copper supplemental level of male yellow-feathered broilers aged from 43 to 63 days is 16 and 32 mg/kg, respectively. Based on the 2. 74 mg/kg copper level in the basal diet, the copper requirement of male yellow-feathered broilers aged from 43 to 63 days is 18.74 and 34.74 mg/kg, respectively.Based on the estimated result of grown performance and liver CuZn-lSOD activity by broken-line regression model, the cop-per requirement of male yellow-feather broilers aged from 43 to 63 days is 19.94 and 38.34 mg/kg, respective-ly.