CAJ | 학술논문

目的：观察乳杆菌A2代谢产物LM1和LM2对人口腔舌鳞癌细胞系Cal-27细胞增殖抑制和诱导凋亡作用。方法：将乳杆菌A2经复原乳清培养基培养制备相应的代谢产物1（LM1），除去代谢产物中的钙离子得到产物2（LM2）；用MTT法和流式细胞仪检测口腔舌鳞状细胞癌的细胞凋亡的情况；琼脂糖凝胶电泳检测 DNA 片段的变化。结果：乳杆菌A2代谢产物LM1和LM2作用Cal-27细胞48 h时，最大抑制率分别为（39.9±1.56）%和（30.5±6.85）%；An-nexinv-FITC/PI染色法检测LM2（8 mg/mL）培养40、44 h时，细胞早期凋亡率分别为19.93%和21.42%；琼脂糖凝胶电泳LM2（8 mg/mL）培养54 h时，在250~750 bp之间显现出典型的“梯”状DNA条带。结论：LM1和LM2能抑制口腔舌鳞癌细胞Cal-27的增殖，呈剂量依赖性关系，LM2能够诱导细胞凋亡。
목적：관찰유간균A2대사산물LM1화LM2대인구강설린암세포계Cal-27세포증식억제화유도조망작용。방법：장유간균A2경복원유청배양기배양제비상응적대사산물1（LM1），제거대사산물중적개리자득도산물2（LM2）；용MTT법화류식세포의검측구강설린상세포암적세포조망적정황；경지당응효전영검측 DNA 편단적변화。결과：유간균A2대사산물LM1화LM2작용Cal-27세포48 h시，최대억제솔분별위（39.9±1.56）%화（30.5±6.85）%；An-nexinv-FITC/PI염색법검측LM2（8 mg/mL）배양40、44 h시，세포조기조망솔분별위19.93%화21.42%；경지당응효전영LM2（8 mg/mL）배양54 h시，재250~750 bp지간현현출전형적“제”상DNA조대。결론：LM1화LM2능억제구강설린암세포Cal-27적증식，정제량의뢰성관계，LM2능구유도세포조망。
Objective:To investigate the effects of Lactobacillus sp. A2′s metabolites on the proliferation and apoptosis of tongue squamous cell caricoma Cal-27 cells. Method: The recovery of whey Lactobacillus medium (whey) was used to prepare the corresponding metabolites of culture 1 (LM1), and being removed the calcium ions metabolites to give the product 2 (LM2). MTT assay was used to test the inhibition effect of LM2 on Cal-27 cells. AnnexinV-FITC/PI staining flow cytometry was used for cell apoptosis. Agarose gel electrophoresis was used to detect the DNA fragments in Cal-27 cells after the treatment by LM2. Results:After Cal-27 cells were treated with LM1 or LM2 for 48 h, maximum inhibition rates were (39.9%±1.56%) and (30.5%±6.85%) respectively. Flow cytometry analysis with annexinV-FITC/PI showed that after treated with LM2 (8 mg/mL) for 40 h and 44 h, cell apoptosis rate was 19.93%and 21.42%. The typical DNA ladders of the treated cells were detected by agarose gel electrophoresis ,when cultured by LM2 (8 mg/mL) for 54 h, the DNA ladders were between 250~750 bp. Conclusion: LM2 may inhibit the proliferation and induce the apoptosis of Cal-27 cells in a dose-dependent manner.