口腔颌面外科杂志
口腔頜麵外科雜誌
구강합면외과잡지
CHINESE JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
2014年
5期
330-335
,共6页
于丽凤%汪安%陈建荣%张磊
于麗鳳%汪安%陳建榮%張磊
우려봉%왕안%진건영%장뢰
碳酸化羟基磷灰石支架%骨形成蛋白-2(BMP-2)%巨噬细胞集落刺激因子(M-CSF)%骨缺损重建%鼠
碳痠化羥基燐灰石支架%骨形成蛋白-2(BMP-2)%巨噬細胞集落刺激因子(M-CSF)%骨缺損重建%鼠
탄산화간기린회석지가%골형성단백-2(BMP-2)%거서세포집락자격인자(M-CSF)%골결손중건%서
carbonated hydroxyapatite scaffold%BMP-2%M-CSF%bone defect reconstruction%rats
目的：通过动物实验检测碳酸化羟基磷灰石支架材料（carbonated hydroxyapatite，CAP）的骨传导性和生物吸收性。并探讨骨形成蛋白-2（rhBMP-2）、重组人巨噬细胞集落刺激因子（rhM-CSF）对CAP成骨特性和生物吸收的影响。方法：选取45只雄性SD大鼠，制备双侧胫骨临界性骨缺损模型，以复合骨形成蛋白-2（rhBMP-2）的碳酸化羟基磷灰石支架材料、复合重组人巨噬细胞集落刺激因子（rhM-CSF）的碳酸化羟基磷灰石支架材料、单纯的碳酸化羟基磷灰石支架材料作为实验组，羟基磷灰石（hydroxyapatite，HAP）作为对照组，植入大鼠胫骨缺损处，并设立空白对照组。术后2、4和8周，通过组织学观察对比新骨形成和材料吸收降解情况。结果：实验组和对照组材料均能完全充填骨缺损，材料界面与骨组织结合紧密，显示了良好的生物相容性和成骨性能。随着植入时间的延长，实验组材料可逐渐降解并被新生骨爬行替代，而对照组未见显著降解和新生骨替代。rhM-CSF能够促进碳酸化羟基磷灰石材料的降解，与CAP组、CAP/rhBMP-2复合物组比较，差异有统计学意义。结论：CAP具有出色的骨传导性和生物吸收性，是一种良好的骨再生移植物。且该支架材料的成骨性和生物降解能够被成骨及破骨细胞因子所调控。
目的：通過動物實驗檢測碳痠化羥基燐灰石支架材料（carbonated hydroxyapatite，CAP）的骨傳導性和生物吸收性。併探討骨形成蛋白-2（rhBMP-2）、重組人巨噬細胞集落刺激因子（rhM-CSF）對CAP成骨特性和生物吸收的影響。方法：選取45隻雄性SD大鼠，製備雙側脛骨臨界性骨缺損模型，以複閤骨形成蛋白-2（rhBMP-2）的碳痠化羥基燐灰石支架材料、複閤重組人巨噬細胞集落刺激因子（rhM-CSF）的碳痠化羥基燐灰石支架材料、單純的碳痠化羥基燐灰石支架材料作為實驗組，羥基燐灰石（hydroxyapatite，HAP）作為對照組，植入大鼠脛骨缺損處，併設立空白對照組。術後2、4和8週，通過組織學觀察對比新骨形成和材料吸收降解情況。結果：實驗組和對照組材料均能完全充填骨缺損，材料界麵與骨組織結閤緊密，顯示瞭良好的生物相容性和成骨性能。隨著植入時間的延長，實驗組材料可逐漸降解併被新生骨爬行替代，而對照組未見顯著降解和新生骨替代。rhM-CSF能夠促進碳痠化羥基燐灰石材料的降解，與CAP組、CAP/rhBMP-2複閤物組比較，差異有統計學意義。結論：CAP具有齣色的骨傳導性和生物吸收性，是一種良好的骨再生移植物。且該支架材料的成骨性和生物降解能夠被成骨及破骨細胞因子所調控。
목적：통과동물실험검측탄산화간기린회석지가재료（carbonated hydroxyapatite，CAP）적골전도성화생물흡수성。병탐토골형성단백-2（rhBMP-2）、중조인거서세포집락자격인자（rhM-CSF）대CAP성골특성화생물흡수적영향。방법：선취45지웅성SD대서，제비쌍측경골림계성골결손모형，이복합골형성단백-2（rhBMP-2）적탄산화간기린회석지가재료、복합중조인거서세포집락자격인자（rhM-CSF）적탄산화간기린회석지가재료、단순적탄산화간기린회석지가재료작위실험조，간기린회석（hydroxyapatite，HAP）작위대조조，식입대서경골결손처，병설립공백대조조。술후2、4화8주，통과조직학관찰대비신골형성화재료흡수강해정황。결과：실험조화대조조재료균능완전충전골결손，재료계면여골조직결합긴밀，현시료량호적생물상용성화성골성능。수착식입시간적연장，실험조재료가축점강해병피신생골파행체대，이대조조미견현저강해화신생골체대。rhM-CSF능구촉진탄산화간기린회석재료적강해，여CAP조、CAP/rhBMP-2복합물조비교，차이유통계학의의。결론：CAP구유출색적골전도성화생물흡수성，시일충량호적골재생이식물。차해지가재료적성골성화생물강해능구피성골급파골세포인자소조공。
Objective: ① To evaluate the osteoconductivity and bioresorbability of the carbonated hydroxyapatite (CAP) scaffold. ②To evaluate the effects of rhBMP-2 and rhM-CSF on bone defect reconstruction with CAP scaffold. Methods:45 male SD rats were comprised in this experiment and randomly divided into 5 groups. A 4 mm ×4 mm critical-size bone defect was created in both sides of tibia in all rats. Group A, CAP was placed into the defect; Group B, placed with CAP/rhBMP-2; Group C, CAP/rhM-CSF was placed; Group D, HAP was placed; and Group E, bone defect left void as control. At 2- week, 4- week and 8-week after restoration, the formation of new bone and the absorption of the materials were ob-served under histological examination. Results: Bone defects were filled completely in experimental groups. The biocom-patiblility was fine. Bone directly bonded to the CAP scaffold. During the native bone remodeling process, the CAP scaf-fold was replaced by new bone as CAP degraded in the experimental groups. But in the HAP group, the decomposition and replacing processes was not significant. rhM-CSF promoted significantly the degradation of carbonated hydroxyapatite scaffold since week 4. Conclusion: ①Carbonated hydroxyapatite scaffold showed good osteoconductivity and bioresorba-biloly in vivo.②rhM-CSF can promote the degradation of carbonated hydroxyapatite scaffold.
