CAJ | 학술논문

采用营养液培养方式，设置0，1．0×10-10和1．0×10-6 mol/L 3个锌处理水平，研究了缺锌胁迫对水稻叶绿体活性氧水平、膜脂过氧化、抗氧化剂含量及抗坏血酸（ AsA）-谷胱甘肽（ GSH）循环系统中相关酶活性的影响．结果表明：1．0×10-10 mol/L锌处理下，水稻叶绿体中H2 O2，O ．-2和丙二醛（ MDA）的含量显著升高，表明缺锌造成了水稻叶绿体内的氧化胁迫；抗坏血酸含量在1．0×10-10 mol/L锌水平上达到最高，而谷胱甘肽含量随缺锌程度的增加呈递减趋势；超氧化物歧化酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶及单脱氢抗坏血酸还原酶等AsA-GSH循环中的关键酶活性均于潜在缺锌（1．0×10-10 mol/L锌）下达到最大值，而在严重缺锌（0 mol/L锌）下又显著下降．研究表明：一定程度上缺锌可诱导水稻叶绿体内AsA-GSH循环系统中的抗氧化剂水平和抗氧化酶活性增加，以减少活性氧的产生与膜脂过氧化的作用；但严重缺锌条件下水稻叶绿体中氧化胁迫突破了抗氧化保护系统的保护能力，使水稻受到伤害．
채용영양액배양방식，설치0，1．0×10-10화1．0×10-6 mol/L 3개자처리수평，연구료결자협박대수도협록체활성양수평、막지과양화、항양화제함량급항배혈산（ AsA）-곡광감태（ GSH）순배계통중상관매활성적영향．결과표명：1．0×10-10 mol/L자처리하，수도협록체중H2 O2，O ．-2화병이철（ MDA）적함량현저승고，표명결자조성료수도협록체내적양화협박；항배혈산함량재1．0×10-10 mol/L자수평상체도최고，이곡광감태함량수결자정도적증가정체감추세；초양화물기화매、항배혈산과양화물매、곡광감태환원매급단탈경항배혈산환원매등AsA-GSH순배중적관건매활성균우잠재결자（1．0×10-10 mol/L자）하체도최대치，이재엄중결자（0 mol/L자）하우현저하강．연구표명：일정정도상결자가유도수도협록체내AsA-GSH순배계통중적항양화제수평화항양화매활성증가，이감소활성양적산생여막지과양화적작용；단엄중결자조건하수도협록체중양화협박돌파료항양화보호계통적보호능력，사수도수도상해．
The rice plants were hydroponically treated with 1.0 ×10 -6(control), 1.0 ×10 -10(potential Zn deficiency) and 0 mol/L Zn ( severe Zn deficiency) .The effects of Zn deficiency on reactive oxygen species, membrane lipid peroxidation, antioxidant contents, and the activities of enzymes in the ascorbic acid ( AsA)-glutathione (GSH) cycle system in the isolated chloroplast were comprehensively investigated.The results showed that contents of H2 O2 , O .-2 and MDA in chloroplasts were significantly increased in response to Zn de-ficiency, implied the severity of oxidant stress.The ascorbic acid content was increased with treatments of po-tential Zn deficiency but markedly decreased when subjected to severe Zn deficiency.Conversely, the contents of glutathione, another important antioxidant in GSH-GSSH cycle, showed a decreasing trend paralleled to the increasing severity of zinc deficiency.The activities of main enzymes in AsA-GSH cycle, including superoxide dismutase ( SOD) , ascorbate peroxidase ( APX) , glutathione reductase ( GR) and monodehydroascorbate re-ductase (MDHAR), were increased by potential Zn deficiency (1.0 ×10 -10 mol/L), but decreased signifi-cantly under severe Zn deficiency ( 0 mol/L ) .The results suggested that a certain extent of Zn deficiency stimulated the capacity of antioxidant systems in chloroplast to reduce reactive oxygen species levels and allevi-ate the peroxidation of membrane lipid, whereas critical Zn deficiency broke down the antioxidant systems in chloroplast, resulting in severe oxidant damage of rice plants.