华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
5期
16-22
,共7页
敖妍%赵磊磊%姜常玉%马履一
敖妍%趙磊磊%薑常玉%馬履一
오연%조뢰뢰%강상옥%마리일
文冠果%miRNA%实时荧光定量PCR%表达分析
文冠果%miRNA%實時熒光定量PCR%錶達分析
문관과%miRNA%실시형광정량PCR%표체분석
Xanthoceras sorbifolia%miRNA%Real-time quantitative PCR%Expression analysis
为了探索miRNA在文冠果不同类型植株的花发育阶段不同组织的表达特异性,以单瓣型和重瓣型文冠果的花芽、茎、叶为试材,提取这2种类型组织的小RNA。从前期研究获得的文冠果小RNA测序数据库中筛选11个候选miRNA,采用实时荧光定量PCR法检测其在花发育不同阶段以及茎和叶片的表达特点。结果表明,11个候选miR-NA具有不同的表达形式。 Xso-M007a-b、miR167a-b与miR169在两类中都是表达量随花芽发育呈现下降趋势,Xso-M003则相反,说明了miRNA表达的时序性。在不同类型之间以及不同部位miRNA的表达也存在差异,Xso-M002、Xso-M003、Xso-M007a-b、miR169、miR319和miR827在两类型花芽中的表达量均高于茎和叶中的表达量。对不同类型、部位和时间点上的表达情况分析,表明miRNA具有明显的品种特异性、组织特异性和时序性。此外,结合miRNA的功能与其表达量差异分析发现,文冠果的形态结构特征与miRNA的表达量变化密切相关。为探索miRNA表达量的变化在林木花器官形成与不同组织分化中的作用提供了重要信息。
為瞭探索miRNA在文冠果不同類型植株的花髮育階段不同組織的錶達特異性,以單瓣型和重瓣型文冠果的花芽、莖、葉為試材,提取這2種類型組織的小RNA。從前期研究穫得的文冠果小RNA測序數據庫中篩選11箇候選miRNA,採用實時熒光定量PCR法檢測其在花髮育不同階段以及莖和葉片的錶達特點。結果錶明,11箇候選miR-NA具有不同的錶達形式。 Xso-M007a-b、miR167a-b與miR169在兩類中都是錶達量隨花芽髮育呈現下降趨勢,Xso-M003則相反,說明瞭miRNA錶達的時序性。在不同類型之間以及不同部位miRNA的錶達也存在差異,Xso-M002、Xso-M003、Xso-M007a-b、miR169、miR319和miR827在兩類型花芽中的錶達量均高于莖和葉中的錶達量。對不同類型、部位和時間點上的錶達情況分析,錶明miRNA具有明顯的品種特異性、組織特異性和時序性。此外,結閤miRNA的功能與其錶達量差異分析髮現,文冠果的形態結構特徵與miRNA的錶達量變化密切相關。為探索miRNA錶達量的變化在林木花器官形成與不同組織分化中的作用提供瞭重要信息。
위료탐색miRNA재문관과불동류형식주적화발육계단불동조직적표체특이성,이단판형화중판형문관과적화아、경、협위시재,제취저2충류형조직적소RNA。종전기연구획득적문관과소RNA측서수거고중사선11개후선miRNA,채용실시형광정량PCR법검측기재화발육불동계단이급경화협편적표체특점。결과표명,11개후선miR-NA구유불동적표체형식。 Xso-M007a-b、miR167a-b여miR169재량류중도시표체량수화아발육정현하강추세,Xso-M003칙상반,설명료miRNA표체적시서성。재불동류형지간이급불동부위miRNA적표체야존재차이,Xso-M002、Xso-M003、Xso-M007a-b、miR169、miR319화miR827재량류형화아중적표체량균고우경화협중적표체량。대불동류형、부위화시간점상적표체정황분석,표명miRNA구유명현적품충특이성、조직특이성화시서성。차외,결합miRNA적공능여기표체량차이분석발현,문관과적형태결구특정여miRNA적표체량변화밀절상관。위탐색miRNA표체량적변화재림목화기관형성여불동조직분화중적작용제공료중요신식。
The aim of this paper is to explore miRNA expression specificities in different flower development stages and tissues in different types of Xanthoceras sorbifolia.Flower buds,stems,and leaves of single and double flower types were taken as materials .High quality small RNAs were isolated from four tissues of the two types .11 candidate miRNAs were chosen from small RNAs data bank of Xanthoceras sorbifolia,and qRT-PCR was used to an-alyze the miRNAs expression in different flower development stages ,stems and leaves .The result showed 11 miRNAs had different expression characters .The expression trends of Xso-M007a-b,miR167a-b and miR169 were down-regula-ted with the flower buds development in two types;on the contrary Xso-M003 was up-regulated .This demonstrated the sequential expression of miRNA .There was also differential expression among different types and tissues .In both types Xso-M002,Xso-M003,Xso-M007a-b,miR169,miR319 and miR827 showed higher expression in flower buds than in stems and leaves .Expression analysis on different types ,tissues and time points proved the miRNA′s expression speci-ficities:species-specificity ,tissue-specificity and time-specificity .In addition , based on miRNA function and expres-sion difference ,it was found that morphological characteristics were closely related to miRNA expression .The paper provided valuable information for exploring the function of miRNA expression variation in forest flower formation and tissues differentiation .