华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
5期
1-4
,共4页
李余生%杨娟%黄胜东%王才林
李餘生%楊娟%黃勝東%王纔林
리여생%양연%황성동%왕재림
水稻%稻曲病%致病菌株%抗病性%数量性状位点
水稻%稻麯病%緻病菌株%抗病性%數量性狀位點
수도%도곡병%치병균주%항병성%수량성상위점
Rice%Rice false smut%Pathogen strains%Disease resistance%Quantitative trait locus
为了鉴定水稻对稻曲病的抗病基因,利用157个家系组成的大关稻( Japonica )/IR28( Indica )重组自交系( Recombinant inbred lines ,RIL)群体,采用人工接种方法,以发病病情指数作为表型值。2012和2013年,接种鉴定亲本及RILs对水稻稻曲病致病菌株Pi-1的抗性。利用QTL Cartographer 分析软件,对水稻抗Pi-1菌株基因进行检测分析。2年共检测到7个QTL,分别为位于第2、7、8、11和12染色体上的qFsr2a、qFsr2b、qFsr7、qFsr8a、qFsr8b、qFsr11、qFsr12,单个位点的贡献率为8.5%~17.2%。其中,2012年检测到qFsr2a、qFsr2b、qFsr8a、qFsr11共4个位点;2013年检测到qFsr7、qFsr8b、qFsr11、qFsr12共4个位点。 qFsr11在2年中均被检测到,对性状的解释率为13.5%和17.2%,作用效应使病情指数下降9.9%和14.3%,提高了抗病性。根据抗性位点加性效应方向,qFsr2a、qFsr8a、qFsr8b、qF-sr11和qFsr12等位点是来自于亲本IR28的增效等位基因,而位点qFsr2b和qFsr7的抗性效应方向相反,是来自于大关稻。稳定遗传的抗病位点qFsr11及其紧密连锁的分子标记可以在分子标记辅助选择育种中得以应用。
為瞭鑒定水稻對稻麯病的抗病基因,利用157箇傢繫組成的大關稻( Japonica )/IR28( Indica )重組自交繫( Recombinant inbred lines ,RIL)群體,採用人工接種方法,以髮病病情指數作為錶型值。2012和2013年,接種鑒定親本及RILs對水稻稻麯病緻病菌株Pi-1的抗性。利用QTL Cartographer 分析軟件,對水稻抗Pi-1菌株基因進行檢測分析。2年共檢測到7箇QTL,分彆為位于第2、7、8、11和12染色體上的qFsr2a、qFsr2b、qFsr7、qFsr8a、qFsr8b、qFsr11、qFsr12,單箇位點的貢獻率為8.5%~17.2%。其中,2012年檢測到qFsr2a、qFsr2b、qFsr8a、qFsr11共4箇位點;2013年檢測到qFsr7、qFsr8b、qFsr11、qFsr12共4箇位點。 qFsr11在2年中均被檢測到,對性狀的解釋率為13.5%和17.2%,作用效應使病情指數下降9.9%和14.3%,提高瞭抗病性。根據抗性位點加性效應方嚮,qFsr2a、qFsr8a、qFsr8b、qF-sr11和qFsr12等位點是來自于親本IR28的增效等位基因,而位點qFsr2b和qFsr7的抗性效應方嚮相反,是來自于大關稻。穩定遺傳的抗病位點qFsr11及其緊密連鎖的分子標記可以在分子標記輔助選擇育種中得以應用。
위료감정수도대도곡병적항병기인,이용157개가계조성적대관도( Japonica )/IR28( Indica )중조자교계( Recombinant inbred lines ,RIL)군체,채용인공접충방법,이발병병정지수작위표형치。2012화2013년,접충감정친본급RILs대수도도곡병치병균주Pi-1적항성。이용QTL Cartographer 분석연건,대수도항Pi-1균주기인진행검측분석。2년공검측도7개QTL,분별위위우제2、7、8、11화12염색체상적qFsr2a、qFsr2b、qFsr7、qFsr8a、qFsr8b、qFsr11、qFsr12,단개위점적공헌솔위8.5%~17.2%。기중,2012년검측도qFsr2a、qFsr2b、qFsr8a、qFsr11공4개위점;2013년검측도qFsr7、qFsr8b、qFsr11、qFsr12공4개위점。 qFsr11재2년중균피검측도,대성상적해석솔위13.5%화17.2%,작용효응사병정지수하강9.9%화14.3%,제고료항병성。근거항성위점가성효응방향,qFsr2a、qFsr8a、qFsr8b、qF-sr11화qFsr12등위점시래자우친본IR28적증효등위기인,이위점qFsr2b화qFsr7적항성효응방향상반,시래자우대관도。은정유전적항병위점qFsr11급기긴밀련쇄적분자표기가이재분자표기보조선택육충중득이응용。
In order to identify the resistant gene of rice false smut in rice ,a recombinant inbred line ( RILs) population with 157 lines derived from an inter-subspecies cross of Daguandao/IR28 by the single seed descent method was used to detect quantitative trait loci ( QTLs)conferring resistance to strain Pi -1 of rice false smut ( caused by Ustilaginoidea virens ( Cooke ) Takahashi ) in 2012 and 2013 .The disease rate index of the two parents and 157 RILs caused by the strain Pi-1 of rice false smut were scored and the QTLs for rice false smut resistance were detected accordingly by QTL Cartographer software .Seven QTLs controlling false smut resistance were detected on chromosomes 2,7,8,11 and 12,respectively,with the phenotypic variance of 8.5%-17.2%.There were four QTLs detected in 2012 and 2013,respectively ,and only one QTL was found in both two years ,the phenotypic variation explained by this individual QTL was 13.5% and 17.2%, and the additive effects of this QTL contributed to the 9.9%and 14.3%decrease of disease index and therefore the disease resistance increased .The direction of the addi-tive effects at five loci qFsr2aq, Fsr8a,qFsr8b,qFsr1 and qFsr12 coincided with that predicted by phenotypes of the parents,and the IR28alleles at these loci had positive effect against rice false smut while the negative effects were found in Daguandao alleles at qFsr2b and qFsr7.The qFsr11 should be useful in rice breeding for resistance to rice false smut in marker-assisted selection (MAS) program.