CAJ | 학술논문

采用体积分数95%乙醇进行加热回流提取后，根据极性由小到大分别用正己烷、二氯甲烷、乙酸乙酯、正丁醇（有机溶剂∶水=1∶1）萃取。利用乙酸乙酯层提取物，经HPLC进一步分离纯化后得到较多的纯馏分。纯化后的馏分经LC-MS进行结构鉴定-定性分析。结果表明：根据质谱信息定性分析馏分1为杨梅素。该方法灵敏、准确、简便。
채용체적분수95%을순진행가열회류제취후，근거겁성유소도대분별용정기완、이록갑완、을산을지、정정순（유궤용제∶수=1∶1）췌취。이용을산을지층제취물，경HPLC진일보분리순화후득도교다적순류분。순화후적류분경LC-MS진행결구감정-정성분석。결과표명：근거질보신식정성분석류분1위양매소。해방법령민、준학、간편。
Using 95% ethanol heated and refluxed for extraction;respectively using hexane,dichloromethane,ethyl acetate,n-butanol according to the polarity from small to large (organic solvent∶water=1∶1).For the sake of more pure fractions,we adopt HPLC to further purification with the extract of ethyl acetate layer.Purified fractions structural identification-qualitative analysis by LC-MS.Based on the mass information carry on qualitative analysis, we found that fraction 1 is myricetin.The method is sensitive,accurate,and convenient.