河南科技学院学报（自然科学版）
河南科技學院學報（自然科學版）
하남과기학원학보（자연과학판）
JOURNAL OF HENAN INSTITUTE OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCES EDITION)
2014年
5期
7-10
,共4页
耐热木聚糖酶%细菌%筛选%鉴定%性质
耐熱木聚糖酶%細菌%篩選%鑒定%性質
내열목취당매%세균%사선%감정%성질
thermostable xylanase%bacteria%screening%identification%characterization
利用木聚糖作为唯一碳源的基础盐培养,在50℃培养条件下,从新乡周围土样及腐殖质中筛选到1株产耐热β-木聚糖酶细菌,初步鉴定为巨大芽孢杆菌（Bacillus megaterium FLH-2）.该菌株在40~55℃生长良好,最适生长温度50℃.木聚糖酶粗酶性质研究表明：该酶最适pH和最适温度分别为6.0和65℃；在pH 5.0~9.0及温度30~75℃之间酶活相对稳定,分别处理30 min,相对酶活仍保持在80%以上.
利用木聚糖作為唯一碳源的基礎鹽培養,在50℃培養條件下,從新鄉週圍土樣及腐殖質中篩選到1株產耐熱β-木聚糖酶細菌,初步鑒定為巨大芽孢桿菌（Bacillus megaterium FLH-2）.該菌株在40~55℃生長良好,最適生長溫度50℃.木聚糖酶粗酶性質研究錶明：該酶最適pH和最適溫度分彆為6.0和65℃；在pH 5.0~9.0及溫度30~75℃之間酶活相對穩定,分彆處理30 min,相對酶活仍保持在80%以上.
이용목취당작위유일탄원적기출염배양,재50℃배양조건하,종신향주위토양급부식질중사선도1주산내열β-목취당매세균,초보감정위거대아포간균（Bacillus megaterium FLH-2）.해균주재40~55℃생장량호,최괄생장온도50℃.목취당매조매성질연구표명：해매최괄pH화최괄온도분별위6.0화65℃；재pH 5.0~9.0급온도30~75℃지간매활상대은정,분별처리30 min,상대매활잉보지재80%이상.
A thermophilic bacterium Bacillus megaterium FLH-2 possessing β-xylanase was isolated from soil and humus samples from Xinxiang and screened at 50 ℃ by using basal medium supplemented with xylan as a sole carbon source.Strain FLH-2 was capable of growing well on 40~55℃and the optimum growth temperature was 50℃. The crude enzyme showed optimum catalytic activity at pH 6.0 and 65 ℃.The enzyme exhibited good pH stability and thermostability,with 80%relativity activity at pH 5.0~9.0 and 30~75℃,respectively.
