河南科技学院学报（自然科学版）
河南科技學院學報（自然科學版）
하남과기학원학보（자연과학판）
JOURNAL OF HENAN INSTITUTE OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCES EDITION)
2014年
5期
1-6
,共6页
孙海燕%付庆云%王朝辉%陈颖
孫海燕%付慶雲%王朝輝%陳穎
손해연%부경운%왕조휘%진영
鬼针草总黄酮%急性肝损伤%丙氨酸氨基转移酶%天冬氨酸氨基转移酶%乳酸脱氢酶
鬼針草總黃酮%急性肝損傷%丙氨痠氨基轉移酶%天鼕氨痠氨基轉移酶%乳痠脫氫酶
귀침초총황동%급성간손상%병안산안기전이매%천동안산안기전이매%유산탈경매
total flavones of Bidens bipinnata L.%acute liver injury%alanine transaminase%aspartate transaminase%lactate dehydrogenase
将66只昆明小鼠随机分为11组：正常组、模型组及鬼针草全草、叶和枝总黄酮治疗组,每治疗组分为低、中和高剂量（40、80、160 mg/kg）亚组,鬼针草总黄酮（TFB）采用正交试验法提取.治疗组连续灌胃14 d后以四氯化碳（CCl4）建立小鼠急性肝损伤模型,IFCC速率法检测小鼠血清中丙氨酸氨基转移酶（alanine transaminase, ALT）、天冬氨酸氨基转移酶（aspartate transaminase,AST）和乳酸脱氢酶（lactate dehydrogenase,LDH）活性.观察不同生理部位TFB对小鼠急性肝损伤时肝功能的影响,探讨其肝损伤保护作用.结果表明：与模型组相比,叶和全草TFB（40 mg/kg）和枝TFB（80 mg/kg）能极显著降低ALT含量（P＜0.01）；仅有叶TFB（40 mg/kg）能极显著降低AST含量（P＜0.01）,而枝TFB（160 mg/kg）能极显著升高AST（P＜0.01）含量；叶和全草TFB在40 mg/kg时LDH活性极显著降低（P＜0.01）.提示鬼针草不同生理部位TFB在急性肝损伤中的作用不同,仅有叶TFB在低剂量（40 mg/kg）时表现出较好的肝保护作用,其余鬼针草各组提示可能肝毒性较大.
將66隻昆明小鼠隨機分為11組：正常組、模型組及鬼針草全草、葉和枝總黃酮治療組,每治療組分為低、中和高劑量（40、80、160 mg/kg）亞組,鬼針草總黃酮（TFB）採用正交試驗法提取.治療組連續灌胃14 d後以四氯化碳（CCl4）建立小鼠急性肝損傷模型,IFCC速率法檢測小鼠血清中丙氨痠氨基轉移酶（alanine transaminase, ALT）、天鼕氨痠氨基轉移酶（aspartate transaminase,AST）和乳痠脫氫酶（lactate dehydrogenase,LDH）活性.觀察不同生理部位TFB對小鼠急性肝損傷時肝功能的影響,探討其肝損傷保護作用.結果錶明：與模型組相比,葉和全草TFB（40 mg/kg）和枝TFB（80 mg/kg）能極顯著降低ALT含量（P＜0.01）；僅有葉TFB（40 mg/kg）能極顯著降低AST含量（P＜0.01）,而枝TFB（160 mg/kg）能極顯著升高AST（P＜0.01）含量；葉和全草TFB在40 mg/kg時LDH活性極顯著降低（P＜0.01）.提示鬼針草不同生理部位TFB在急性肝損傷中的作用不同,僅有葉TFB在低劑量（40 mg/kg）時錶現齣較好的肝保護作用,其餘鬼針草各組提示可能肝毒性較大.
장66지곤명소서수궤분위11조：정상조、모형조급귀침초전초、협화지총황동치료조,매치료조분위저、중화고제량（40、80、160 mg/kg）아조,귀침초총황동（TFB）채용정교시험법제취.치료조련속관위14 d후이사록화탄（CCl4）건립소서급성간손상모형,IFCC속솔법검측소서혈청중병안산안기전이매（alanine transaminase, ALT）、천동안산안기전이매（aspartate transaminase,AST）화유산탈경매（lactate dehydrogenase,LDH）활성.관찰불동생리부위TFB대소서급성간손상시간공능적영향,탐토기간손상보호작용.결과표명：여모형조상비,협화전초TFB（40 mg/kg）화지TFB（80 mg/kg）능겁현저강저ALT함량（P＜0.01）；부유협TFB（40 mg/kg）능겁현저강저AST함량（P＜0.01）,이지TFB（160 mg/kg）능겁현저승고AST（P＜0.01）함량；협화전초TFB재40 mg/kg시LDH활성겁현저강저（P＜0.01）.제시귀침초불동생리부위TFB재급성간손상중적작용불동,부유협TFB재저제량（40 mg/kg）시표현출교호적간보호작용,기여귀침초각조제시가능간독성교대.
To investigate the protective effect of total flavones of bidens bipinnata L. (TFB) on acute liver injury, sixty and six mouse were randomly divided into 11 groups:normal group,model group,and low,medium and high-dosage groups of leaf,branch and whole plant TFB.Acute liver injury was induced by CCl4 injected intraperitoneally after last administration of TFB for 2 weeks.Serum ALT,AST and LDH were examined by IFCC.The results showed that:compared with model group,leaf and whole plant TFB groups with low dose (40 mg/kg) and stem TFB with 80 mg/kg significantly inhibited the activities of ALT (P<0.01). Only leaf TBF with 40 mg/kg was observed to decreased AST level (P<0.01),whereas stem TBF with 160 mg/kg significantly increased AST level (P<0.01).As for LDH activity,lower level was observed at leaf and whole plant TFBwith 40 mg/kg groups.Different physiological parts of TFB has the different protective effect on acute liver injury,showing that leaf TFB at lower level has stronger protective effect.However,higher TBF dosage might have hepatotoxicity.
