CAJ | 학술논문

高浓度壳聚糖溶液制备得到粒径较小和均一的微粒，应用于食品的防腐与杀菌。采用壳聚糖在植物油与水的两相溶剂体系中与三聚磷酸钠(TPP)进行交联制备得到微粒，通过单因素实验确定其最适制备条件。然后通过其对大肠杆菌和金黄色葡萄球菌的杀菌实验研究其抑菌性能。当壳聚糖溶液的浓度为12 g?L-1时，可以制备得到平均粒径为(111.3±1.06) nm，PDI值为0.21±0.011。抑菌实验结果表明，当壳聚糖微粒浓度为125μg?mL-1时，壳聚糖微粒对大肠杆菌和金黄色葡萄球菌的杀菌率分别达到90.54%和91.34%。高浓度壳聚糖可以在植物油与水的两相溶剂体系中制备得到粒径较小和均一的微粒，并具有很好的杀菌活性。
고농도각취당용액제비득도립경교소화균일적미립，응용우식품적방부여살균。채용각취당재식물유여수적량상용제체계중여삼취린산납(TPP)진행교련제비득도미립，통과단인소실험학정기최괄제비조건。연후통과기대대장간균화금황색포도구균적살균실험연구기억균성능。당각취당용액적농도위12 g?L-1시，가이제비득도평균립경위(111.3±1.06) nm，PDI치위0.21±0.011。억균실험결과표명，당각취당미립농도위125μg?mL-1시，각취당미립대대장간균화금황색포도구균적살균솔분별체도90.54%화91.34%。고농도각취당가이재식물유여수적량상용제체계중제비득도립경교소화균일적미립，병구유흔호적살균활성。
Chitosan particulates with small size and uniform dispersion were prepared for food anticorrosion and sterilization using high chitosan concentration solutions. These particulates were prepared using chitosan solution and sodium tripolyphosphate (TPP) via ionic crosslinking in oil/water two-phase solvent system. The optimal condition for the preparation was determined by single factor tests and the antimicrobial activities were evaluated with Escherichia coli, Staphylococcus aureus in vitro. When the concentration of the chitosan solution is 12 g?L-1, the mean diameter of chitosan particulates prepared is (111.3±1.06) nm and the PDI is 0.21±0.011. Antibacterial results show that when the concentration of chitosan particulates is 125μg?mL-1, the antibacterial activity against Escherichia coli and Staphylococcus aureus are 90.54% and 91.34%, respectively. The high concentration of chitosan leads to chitosan particulates with small size and uniform dispersion prepared in the two-phase solvent system and the results show that the chitosan particulates prepared have high antibacterial activity.