CAJ | 학술논문

采用重组大肠杆菌 Lin 43利用甘油产丙酮醇。为了改善菌株对丙酮醛的耐受性，采取敲除 glo B基因而不是gloA 基因以阻断丙酮醛的脱毒途径。实验结果表明，菌株 Lin43ΔgloB 对丙酮醛的耐受性要明显优于 Lin43ΔgloA。在含20 g?L-1的甘油磷酸盐缓冲液中，通过26 h 静息细胞转化，菌株 Lin43?gloB pCA24N-yqhE 丙酮醇产量达到2.30 g?L-1，并能耐受3.5 mmol?L-1的丙酮醛。同时 Lin43?gloB pCA24N-yqhE 实现重复发酵，第二轮发酵的产量可达1.53 g?L-1，丙酮醇的得率与第一轮相同。
채용중조대장간균 Lin 43이용감유산병동순。위료개선균주대병동철적내수성，채취고제 glo B기인이불시gloA 기인이조단병동철적탈독도경。실험결과표명，균주 Lin43ΔgloB 대병동철적내수성요명현우우 Lin43ΔgloA。재함20 g?L-1적감유린산염완충액중，통과26 h 정식세포전화，균주 Lin43?gloB pCA24N-yqhE 병동순산량체도2.30 g?L-1，병능내수3.5 mmol?L-1적병동철。동시 Lin43?gloB pCA24N-yqhE 실현중복발효，제이륜발효적산량가체1.53 g?L-1，병동순적득솔여제일륜상동。
Gene gloB instead of gloA in the strain of Escherichia coli Lin43 was knocked out to disrupt the detoxification pathway of methylglyoxal. The results show that the tolerability of strain Lin43ΔgloB is much better than that of the strain Lin43 ΔgloA. After resting the cells of strain Lin43 ΔgloB pCA24N-yqhE in the phosphate buffer with 20 g?L-1of glycerol, it produces 2.30 g?L-1 of acetol in 26 h and can resist 3.5 mmol?L-1 methylglyoxal. Moreover, 1.53 g?L-1 acetol can be produced with strain Lin43 ΔgloB pCA24N-yqhE in the second round production, which has similar yield as that of the first round.