CAJ | 학술논문

为了研究条斑紫菜TPS基因（ PyTPS）对花生种子耐盐性和品质性状的影响，利用花粉管通道法，将携带条斑紫菜PyTPS基因的pCAMBIA2300-PyTPS重组载体导入花生，获得了T0转基因植株。利用PCR对T0植株进行了检测，PCR阳性率为34．7％。将部分转基因植株的T2种子进行耐盐试验，利用半定量RT-PCR分析了PyTPS基因在转基因植株中的表达情况，并用近红外技术测定了T2种子的品质性状。结果表明，部分转基因花生种子在盐溶液中的发芽率显著提高，PyTPS基因可在有些转基因植株中表达，有的转基因植株的蛋白质、油酸、亚油酸或脂肪含量发生了明显变化。总之，条斑紫菜PyTPS基因导入花生后，既可提高转基因花生种子的耐盐性，又对部分后代种子的品质性状产生影响。
위료연구조반자채TPS기인（ PyTPS）대화생충자내염성화품질성상적영향，이용화분관통도법，장휴대조반자채PyTPS기인적pCAMBIA2300-PyTPS중조재체도입화생，획득료T0전기인식주。이용PCR대T0식주진행료검측，PCR양성솔위34．7％。장부분전기인식주적T2충자진행내염시험，이용반정량RT-PCR분석료PyTPS기인재전기인식주중적표체정황，병용근홍외기술측정료T2충자적품질성상。결과표명，부분전기인화생충자재염용액중적발아솔현저제고，PyTPS기인가재유사전기인식주중표체，유적전기인식주적단백질、유산、아유산혹지방함량발생료명현변화。총지，조반자채PyTPS기인도입화생후，기가제고전기인화생충자적내염성，우대부분후대충자적품질성상산생영향。
In this experiment,to analyze the effects of PyTPS from Porphyra yezoensis on the salt tolerance and quality characteristics of seed of peanut ,the recombinant expression vector pCAMBIA 2300-PyTPS harboring PyTPS was transformed into peanut via pollen-tube pathway method .Transgenic plants of T 0 generation were analyzed by PCR and the positive rate was 34.7%.Transgenic seeds of T2 generation were tested for salt tolerance in a 0.7%NaCl solution and for quality by near infrared spectroscopy ,and the expression level of PyTPS gene in transgenic plants was analyzed by semi-quantity RT-PCR.These results suggested that the germination rate was substantially higher for the seeds from some transgenic plants than for seeds from the non-transgenic parent in the NaCl solution and transcription of PyTPS gene was detected in some transgenic plants .Significant changes were found in quality characteristics including protein content , oleic acid and linoleic acid content , oil content of some T 2-generation transgenic seeds .In a word,transgenic seeds of peanut exhibited enhanced tolerance to salinity stress and significant variation in quality characteristics after ectopic expression of the PyTPS gene in peanut .