CAJ | 학술논문

为阐明光照和温度对烟草西柏三烯二醇合成的调控机制，以烟草突变品系8306及其原始株12451为材料，采用气相色谱-质谱联用技术（ GC-MS）和实时荧光定量PCR技术，研究了不同昼夜温差和不同光照强度条件下烟草西柏三烯二醇的合成差异以及烟草CYC-1和CYP71D16基因的表达差异。结果表明，昼夜温差15℃条件下西柏三烯二醇合成量以及CYC-1和CYP71D16基因表达量明显高于昼夜温差0℃处理，说明较大的昼夜温差促进CYC-1和CYP71D16基因上调表达，从而使西柏三烯二醇得到充分的积累；光照强度100000 lx条件下西柏三烯二醇合成量以及CYC-1和CYP71D16基因表达量明显高于光照强度40000 lx处理，说明光照不足抑制了CYC-1和CYP71D16的表达，较高的光照强度有利于西柏三烯二醇的合成与积累。
위천명광조화온도대연초서백삼희이순합성적조공궤제，이연초돌변품계8306급기원시주12451위재료，채용기상색보-질보련용기술（ GC-MS）화실시형광정량PCR기술，연구료불동주야온차화불동광조강도조건하연초서백삼희이순적합성차이이급연초CYC-1화CYP71D16기인적표체차이。결과표명，주야온차15℃조건하서백삼희이순합성량이급CYC-1화CYP71D16기인표체량명현고우주야온차0℃처리，설명교대적주야온차촉진CYC-1화CYP71D16기인상조표체，종이사서백삼희이순득도충분적적루；광조강도100000 lx조건하서백삼희이순합성량이급CYC-1화CYP71D16기인표체량명현고우광조강도40000 lx처리，설명광조불족억제료CYC-1화CYP71D16적표체，교고적광조강도유리우서백삼희이순적합성여적루。
Using 8306 and 12451 tobacco cultivars as the materials ,the effects of temperature range between day and night and light intensity on the content of CBT-diols and the expression of CYC-1 and CYP71D16 were studied . When the temperature range was 15 ℃,the content of CBT-diols and the expression levels of CYC-1 and CYP71D16 were significant higher than those in the temperature range of 0 ℃.This suggests that larger temperature range could promote the up-regulated expression of CYC-1 and CYP71D16,so the CBT-diols accumulated more sufficiently .In the condition of 100 000 lx light intensity ,the content of CBT-diols and the expression levels of CYC-1 and CYP71D16 were significantly higher than those in the condition of 40 000 lx light intensity .This suggests that shading inhibited the expression of CYC-1 and CYP71D16 and larger light intensity promoted the synthesis of CBT-diols.