华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2014年
5期
139-143
,共5页
陈波%张燕%曹墨菊%荣廷昭
陳波%張燕%曹墨菊%榮廷昭
진파%장연%조묵국%영정소
玉米%根缘细胞%NaCl胁迫%萌发期
玉米%根緣細胞%NaCl脅迫%萌髮期
옥미%근연세포%NaCl협박%맹발기
Maize%Root border cell%NaCl stress%Germination stage
NaCl胁迫是导致玉米盐害的主要因素,研究了NaCl对玉米萌发期根系和离体根缘细胞的影响。结果表明,随着NaCl浓度从0 mmol/L增加到250 mmol/L,胚根长和次生根数量受到显著抑制,同时根缘细胞存活率也显著降低。但在100 mmol/L的NaCl胁迫下,根缘细胞的数量比对照和高浓度胁迫有显著增加,同时100 mmol/L的NaCl胁迫也显著刺激了玉米离体根缘细胞的凋亡速度。由于试验材料对100 mmol/L的NaCl胁迫有一定耐性,因此,认为该胁迫浓度下根缘细胞数量增加可能与其耐盐性有关,为进一步探讨根缘细胞与玉米耐盐机制的关系奠定了基础。
NaCl脅迫是導緻玉米鹽害的主要因素,研究瞭NaCl對玉米萌髮期根繫和離體根緣細胞的影響。結果錶明,隨著NaCl濃度從0 mmol/L增加到250 mmol/L,胚根長和次生根數量受到顯著抑製,同時根緣細胞存活率也顯著降低。但在100 mmol/L的NaCl脅迫下,根緣細胞的數量比對照和高濃度脅迫有顯著增加,同時100 mmol/L的NaCl脅迫也顯著刺激瞭玉米離體根緣細胞的凋亡速度。由于試驗材料對100 mmol/L的NaCl脅迫有一定耐性,因此,認為該脅迫濃度下根緣細胞數量增加可能與其耐鹽性有關,為進一步探討根緣細胞與玉米耐鹽機製的關繫奠定瞭基礎。
NaCl협박시도치옥미염해적주요인소,연구료NaCl대옥미맹발기근계화리체근연세포적영향。결과표명,수착NaCl농도종0 mmol/L증가도250 mmol/L,배근장화차생근수량수도현저억제,동시근연세포존활솔야현저강저。단재100 mmol/L적NaCl협박하,근연세포적수량비대조화고농도협박유현저증가,동시100 mmol/L적NaCl협박야현저자격료옥미리체근연세포적조망속도。유우시험재료대100 mmol/L적NaCl협박유일정내성,인차,인위해협박농도하근연세포수량증가가능여기내염성유관,위진일보탐토근연세포여옥미내염궤제적관계전정료기출。
NaCl stress is the major factor in salt damage to maize .In this paper ,we have studied on the effects of NaCl on the growth of maize roots and root border cells in vitro in germination stage .The results showed that ,with the increase of NaCl concentration , radicle length and the number of secondary roots was significantly inhibited , while the survival rate of root border cells was also significantly reduced .However , compared to the control and higher concentration ,under the stress of 50-100 mmol/L NaCl ,the number of border cells significantly increased , while the stress of 100 mmol/L NaCl maize also significantly stimulated apoptosis rate of border cells in vitro.It in-dicated the increasing of the number of root border cells and accelerating of apoptosis may be related to the toler -ance of maize to lower concentration NaCl stress .This will provide a theoretical basis for in-depth study on maize salt tolerance mechanism ,from the perspective of root border cells responding .