CAJ | 학술논문

为研究盐胁迫对小麦代换系酶活性的影响，以中国春-Synthetic 6x 染色体代换系及其亲本为材料，通过测定在盐处理条件下幼苗抗氧化酶SOD、POD活性和丙二醛（ MDA）含量变化，并对其相关耐盐特性的基因进行染色体定位。采用霍格兰营养液水培法，设置对照（0 mmol/L NaCl）和盐处理（150 mmol/L NaCl），在幼苗两叶一心时进行处理，四叶一心时取样，分别测定对照和盐处理条件下幼苗的SOD和POD活性以及MDA含量。在盐胁迫条件下，小麦代换系幼苗SOD和POD活性显著升高，丙二醛含量降低。其中，1A、5A、6A、1B、5B、6B、7B和5D代换系的SOD活性显著或极显著高于母本中国春，2A、1B、2B、3B、5B、6B、5D和6D代换系的相对SOD 活性显著或极显著高于母本中国春；3A、4A、5A、6A、7A、6B和7D代换系的POD 活性显著或极显著高于中国春，4A、5A、6B、1D和7D 代换系的相对POD活性显著或极显著高于中国春。由于保护酶的作用，在盐胁迫条件下，多数代换系MDA含量明显减少，2A、3A、4A、6A、1B、3B、4B、5B、6B、7B、1D、5D和7D代换系的MDA含量显著或极显著低于母本中国春，2A、6A、6B、1D和2D代换系的相对MDA含量显著或极显著低于母本中国春。 Synthetic 6x 的1B、5B、6B 和5D 染色体上可能存在诱导幼苗SOD活性增强的基因，4A、5A、6B和7D染色体上可能存在诱导幼苗POD 活性增强的基因，抑制幼苗MDA含量增高的基因可能存在于2A、6A、6B和1D染色体上。
위연구염협박대소맥대환계매활성적영향，이중국춘-Synthetic 6x 염색체대환계급기친본위재료，통과측정재염처리조건하유묘항양화매SOD、POD활성화병이철（ MDA）함량변화，병대기상관내염특성적기인진행염색체정위。채용곽격란영양액수배법，설치대조（0 mmol/L NaCl）화염처리（150 mmol/L NaCl），재유묘량협일심시진행처리，사협일심시취양，분별측정대조화염처리조건하유묘적SOD화POD활성이급MDA함량。재염협박조건하，소맥대환계유묘SOD화POD활성현저승고，병이철함량강저。기중，1A、5A、6A、1B、5B、6B、7B화5D대환계적SOD활성현저혹겁현저고우모본중국춘，2A、1B、2B、3B、5B、6B、5D화6D대환계적상대SOD 활성현저혹겁현저고우모본중국춘；3A、4A、5A、6A、7A、6B화7D대환계적POD 활성현저혹겁현저고우중국춘，4A、5A、6B、1D화7D 대환계적상대POD활성현저혹겁현저고우중국춘。유우보호매적작용，재염협박조건하，다수대환계MDA함량명현감소，2A、3A、4A、6A、1B、3B、4B、5B、6B、7B、1D、5D화7D대환계적MDA함량현저혹겁현저저우모본중국춘，2A、6A、6B、1D화2D대환계적상대MDA함량현저혹겁현저저우모본중국춘。 Synthetic 6x 적1B、5B、6B 화5D 염색체상가능존재유도유묘SOD활성증강적기인，4A、5A、6B화7D염색체상가능존재유도유묘POD 활성증강적기인，억제유묘MDA함량증고적기인가능존재우2A、6A、6B화1D염색체상。
Wheat substitution lines between Chinese Spring and Synthetic 6x under the treatments of salt stress were studied to research the effect on antioxidant enzymes SOD ,POD activities and MDA content and locating the gene controlling antioxidant enzymes SOD , POD activities and MDA content .Two control groups were carried out under hydroponic experiment .The first control group was growing under Hoagland′s solution(0 mmol/L NaCl).The second control group was growing under Hoagland′s solution with 150 mmol/L NaCl.Seedlings was treated at the two-leaf stage and measured SOD and POD activity and MDA content under control and salt treatment conditions at four-leaf stage .Substitution lines of wheat significantly increased of SOD and POD activity under salt stress condi -tions,but the MDA content decreased .SOD activity of 1A,5A,6A,1B,5B,6B,7B and 5D substitution lines were significantly or very significantly higher than female Chinese Spring .2A,1B,2B,3B,5B,6B,5D and 6D substitution lines of relative SOD activity was significantly or very significantly higher than female Chinese Spring;POD activity of 3A,4A,5A,6A,7A,6B and 7D substitution lines were significantly or very significantly higher than China Spring,4A,5A,6B,1D and 7D substitution lines of relative POD activity significantly or very significantly higher than Chinese Spring .As the role of protective enzymes under salt stress conditions ,the MDA content of substitution lines was significantly reduced .2A,3A,4A,6A,1B,3B,4B,5B,6B,7B,1D,5D and 7D substitution lines of MDA content were significantly or very significantly lower than female Chinese Spring ,2A,6A,6B,1D and 2D substitution lines of relative MDA content significantly or very significantly lower than female Chinese Spring .The results showed that the genes increased SOD activity and POD activity might be located on 1B,5B,6B and 5D chromosome and 4A,5A,6B and 7D chromosome of Synthetic 6x respectively;while the genes inhibiting content might be exist in 2A,6A,6B and 1D chromosome of Synthetic 6x under salt stress.