CAJ | 학술논문

通过对不同萝卜雄性不育细胞质进行鉴定与分类，确定不育细胞质的类型，为更好地利用这些不育细胞质提供科学依据。利用5个来源不同的萝卜细胞质雄性不育材料和8个可育的白萝卜品种，从恢保关系、花粉败育方式及线粒体DNA的分子标记等3个方面对其进行了研究。结果发现，根据5个细胞质雄性不育的恢保关系，可将其分为2种细胞质类型。进一步对其花器官形态及花粉败育方式研究发现，类型1花丝短缩，花药瘦小，败育从四分体时期开始，表现为绒毡层细胞液泡化，最终药室的基本结构消失，内无花粉粒；类型2花药大小与可育相似，雄蕊稍低于柱头，败育从单核小孢子时期开始，能够保持药室的基本结构，药室内存在没有活力的花粉粒外壳。 PCR检测结果表明：类型1为Ogura不育细胞质类型，类型2为NWB不育细胞质类型。
통과대불동라복웅성불육세포질진행감정여분류，학정불육세포질적류형，위경호지이용저사불육세포질제공과학의거。이용5개래원불동적라복세포질웅성불육재료화8개가육적백라복품충，종회보관계、화분패육방식급선립체DNA적분자표기등3개방면대기진행료연구。결과발현，근거5개세포질웅성불육적회보관계，가장기분위2충세포질류형。진일보대기화기관형태급화분패육방식연구발현，류형1화사단축，화약수소，패육종사분체시기개시，표현위융전층세포액포화，최종약실적기본결구소실，내무화분립；류형2화약대소여가육상사，웅예초저우주두，패육종단핵소포자시기개시，능구보지약실적기본결구，약실내존재몰유활력적화분립외각。 PCR검측결과표명：류형1위Ogura불육세포질류형，류형2위NWB불육세포질류형。
The classification of different male sterile cytoplasms can provide guide to the utilization of it .In this study,we have investigated five cytoplasm male steriles ( CMS) of radish.The five cytoplasm male sterile lines were crossed with 8 fertile breeding lines ,and the fertility of the F 1 progenies was different .According to the fertility of the F1 progenies,the five cytoplasms were classified into two types .Cytological examination of pollen development stages showed a clear difference in the defective stage from that seen in the two types of male sterility .At the tetrad stage,the tapetum cells of type 1 CMS was bulged by severe cytoplasmic vacuolation ,but no significant differences were observed in type 2 CMS during this stage .After the microspores were released ,microspores of the type 1 CMS began to degenerate finally resulting in empty anthers without pollen grains .However ,the microspores of the type 2 CMS plant failed to develop into mature pollen grains .The size of the just-released microspores and the just-before-dehiscence stage microspores remained unchanged .PCR analysis revealed that the type 1 was Ogura CMS and the other was NWB CMS .