CAJ | 학술논문

为了明确聚乙二醇（ PEG-8000）模拟干旱胁迫对马铃薯组培苗的影响，以11个马铃薯主栽品种的脱毒组培苗为试验材料，研究比较了在固体培养基中分别添加5％，10％，15％，20％的 PEG-8000模拟干旱胁迫和对照处理下（不含PEG-8000）组培苗生长指标的变化，对供试品种组培苗的株高、最大根长、茎鲜质量、根鲜质量、茎干质量、根干质量和植物水分含量共7个生长指标进行测定和分析。结果表明，随着胁迫处理PEG-8000浓度的增加，所有材料的组培苗表现出受干旱胁迫现象越严重，当固体培养基中PEG-8000浓度为15％时，11个品种间的差异最大，所测的7个指标值均显著低于对照，是鉴定马铃薯品种组培苗对干旱胁迫反应的最佳浓度。在该胁迫浓度下，供试马铃薯材料的平均相对根鲜质量为26．9％，变异系数为50．6％，是下降幅度和变异系数最大的一个指标，表明根鲜质量更易受到干旱胁迫的影响。利用6个相对指标值，对11个马铃薯品种的干旱敏感性进行聚类分析，在欧氏距离为40处，11个马铃薯品种可分为3类，Ⅰ类包括坝薯10号、高原7号、克新1号和晋薯2号；Ⅱ类包括中薯2号和中薯20号；Ⅲ类包括中薯3号、大西洋、夏坡蒂、费乌瑞它和疫不加，3个类群受干旱胁迫的影响逐渐加重。综合聚类分析和植株含水量的结果，筛选到坝薯10号和高原7号2个耐旱品种。
위료명학취을이순（ PEG-8000）모의간한협박대마령서조배묘적영향，이11개마령서주재품충적탈독조배묘위시험재료，연구비교료재고체배양기중분별첨가5％，10％，15％，20％적 PEG-8000모의간한협박화대조처리하（불함PEG-8000）조배묘생장지표적변화，대공시품충조배묘적주고、최대근장、경선질량、근선질량、경간질량、근간질량화식물수분함량공7개생장지표진행측정화분석。결과표명，수착협박처리PEG-8000농도적증가，소유재료적조배묘표현출수간한협박현상월엄중，당고체배양기중PEG-8000농도위15％시，11개품충간적차이최대，소측적7개지표치균현저저우대조，시감정마령서품충조배묘대간한협박반응적최가농도。재해협박농도하，공시마령서재료적평균상대근선질량위26．9％，변이계수위50．6％，시하강폭도화변이계수최대적일개지표，표명근선질량경역수도간한협박적영향。이용6개상대지표치，대11개마령서품충적간한민감성진행취류분석，재구씨거리위40처，11개마령서품충가분위3류，Ⅰ류포괄패서10호、고원7호、극신1호화진서2호；Ⅱ류포괄중서2호화중서20호；Ⅲ류포괄중서3호、대서양、하파체、비오서타화역불가，3개류군수간한협박적영향축점가중。종합취류분석화식주함수량적결과，사선도패서10호화고원7호2개내한품충。
To know the effect of drought stress induced by polyethylene glycol 8000 ( PEG-8000 ) on growth in-dicators of potato plantlets in vitro,eleven main potato varieties in China were evaluated by adding 5%,10%,15%and 20%( W∶V) of PEG-8000 to Murashige-Skoog solid medium respectively with do not contain PEG-8000 as the control.Seven characters:plant height,root length,shoot fresh weight,root fresh weight,shoot dry weight,root dry weight and plant water content ,were measured and analyzed .The results indicated that all potato plantlets in vitro showed more severe drought stress , when facing with increasing concentration of PEG .Under 15% PEG-8000 stress,the eleven varieties showed the largest differences and all growth indicators were lower than that in control condition ,therefore 15%PEG-8000 was the best concentration to simulate drought stress for potato plantlets in vitro. Under such stress ,the relative values of indicators were also analyzed ,the results showed that the average of relative root fresh weight was 26.9%,was decreased most and had the largest variation coefficient of50 6.%,the relative root fresh weight was the most sensitive to drought stress among all indicators .By clustering analysis ,the 11 cultivars were divided into 3 groups at Euclidean distance of 40.The effects induced by drought stress among the three groups are gradually increased:groupⅠcontains Bashu 10 ,Gaoyuan 7 ,Kexin 1 and Jinshu 2;groupⅡcontains Zhongshu 2 and Zhongshu 20;group Ⅲ contains Zhongshu 3,Atlantic,Favorita and Epoka .After clustering analysis and plant water content analysis ,Bashu 10 and Gaoyuan 7 could be classified as drought tolerant varieties .