CAJ | 학술논문

为建立鸡小肠上皮干细胞（Intestinal epithelial stem cells，IECs）体外培养体系并对其进行生物学特性研究，取18日龄鸡胚，分离小肠获得IECs，在DMEM/F12培养基中培养并观察其形态。通过传代及生长曲线的绘制分析其增殖情况，并经形态学观察及细胞免疫组织化学、RT-PCR等方法对所得到的纯化后的鸡小肠上皮干细胞进行鉴定。结果表明，鸡IECs可在体外扩增培养，目前传代至P11，纯化所得的鸡小肠上皮细胞经形态学观察及免疫荧光、RT-PCR法鉴定为阳性，由此可得出结论：鸡小肠组织中可以分离出IECs，且能够在体外增殖培养。
위건립계소장상피간세포（Intestinal epithelial stem cells，IECs）체외배양체계병대기진행생물학특성연구，취18일령계배，분리소장획득IECs，재DMEM/F12배양기중배양병관찰기형태。통과전대급생장곡선적회제분석기증식정황，병경형태학관찰급세포면역조직화학、RT-PCR등방법대소득도적순화후적계소장상피간세포진행감정。결과표명，계IECs가재체외확증배양，목전전대지P11，순화소득적계소장상피세포경형태학관찰급면역형광、RT-PCR법감정위양성，유차가득출결론：계소장조직중가이분리출IECs，차능구재체외증식배양。
This current study was conducted to investigate the biological characteristics of chicken IECs ( Intes-tinal epithelial stem cells)by isolating and culturing in vitro.IECs were isolated from small intestine of 18 day-old chicken embryos .The suitable culture medium for IECs was DMEM/F12 .Then its proliferation was analyzed by subculture and the growth curve .The purified chicken intestinal epithelial stem cells were identified by morphology , immunohistochemical methods and RT-PCR.The results showed that chicken IECs can be cultured in vitro and have been passed to 11 generation .The purified IECs were positive by morphology , immunohistochemical methods and RT-PCR.To sum up,IECs can be isolated from small intestine ,which can proliferate rapidly in vitro.