CAJ | 학술논문

微纳流量的测量是微纳液相色谱仪研发工作中的关键环节之一，本文利用热分布原理，建立了一种可以实时测量流量的微流量计法。由分流模式获得微纳流量流体，再采用传统测量方法得到实际输出流量，用以校正微流量计测量法的准确性。结果表明：微流量计法测得的数据准确且稳定性好，能够真实反映瞬时流量的变化，可测量μL /min ～ nL/min 范围。该方法的建立，为微纳输液泵研制提供了有效的测试手段。
미납류량적측량시미납액상색보의연발공작중적관건배절지일，본문이용열분포원리，건립료일충가이실시측량류량적미류량계법。유분류모식획득미납류량류체，재채용전통측량방법득도실제수출류량，용이교정미류량계측량법적준학성。결과표명：미류량계법측득적수거준학차은정성호，능구진실반영순시류량적변화，가측량μL /min ～ nL/min 범위。해방법적건립，위미납수액빙연제제공료유효적측시수단。
Testing for micro-nano flow rate is one of important works in the μ-HPLC’s development . A new micro-flow rate meter testing method was proposed and evaluated .Its principle was to acquire liq-uid’s instant flow rate by monitoring the temperature change of the fluid .The flow rate was splited by split ratio to achieve the required micro-nano flow rate .The micro-flow rate meter was calibrated by tradi-tional testing method .The results indicated that the micro-flow rate meter testing method possessed better accuracy and repeatability .Besides ,the new method could measure accurate instant flow rate accurately , and the range was μL/min to nL /min .This new method provided an effective way to design and improve micro-nano pumps .