CAJ | 학술논문

目的：建立高效液相色谱法测定贝那普利氢氯噻嗪片的含量。方法采用 Ultimate AQ-C18柱（250 mm ×4．6 mm ，5μm）；以四氢呋喃-水-冰醋酸（250 mL ＋750 mL ＋4 mL 加入四丁基溴化铵1．0 g）为流动相，检测波长：240 nm ；流速：1．5 mL ? min -1。结果盐酸贝那普利质量浓度在41．2～412．3μg ? mL -1范围内呈线性，r＝0．9995，氢氯噻嗪质量浓度在51．0～509．6μg ? mL -1范围内呈线性，r＝0．9998；盐酸贝那普利平均回收率在99．6％～101．6％之间，RSD 小于2％，氢氯噻嗪平均回收率在99．3％～100．7％之间，RSD 小于2％。结论该方法操作简便、准确、灵敏、重复性好，可用于贝那普利氢氯噻嗪片的质量控制。
목적：건립고효액상색보법측정패나보리경록새진편적함량。방법채용 Ultimate AQ-C18주（250 mm ×4．6 mm ，5μm）；이사경부남-수-빙작산（250 mL ＋750 mL ＋4 mL 가입사정기추화안1．0 g）위류동상，검측파장：240 nm ；류속：1．5 mL ? min -1。결과염산패나보리질량농도재41．2～412．3μg ? mL -1범위내정선성，r＝0．9995，경록새진질량농도재51．0～509．6μg ? mL -1범위내정선성，r＝0．9998；염산패나보리평균회수솔재99．6％～101．6％지간，RSD 소우2％，경록새진평균회수솔재99．3％～100．7％지간，RSD 소우2％。결론해방법조작간편、준학、령민、중복성호，가용우패나보리경록새진편적질량공제。
Objective To establish an HPLC method for the determination of Benazepril Hydrochloride and Hydrochlorothiazide Tablets .Methods The chromatographic conditions were as follows :Ultimate AQ-C18 column (250 mm × 4 .6 mm 5 μm) ,mobile phase of butylene oxide-water-glacial acetic acid (250 mL + 750 mL + 4 mL ,add tetrabutylammonium bromide 1 .0 g ) ,detection wavelength at 240 nm ,and flow rate 1 .5 mL ? min - 1 .Results The calibration curve of benazepril hydrochloride was linear in the range of 41 .2-412 .3 μg ? mL - 1 ,r= 0 .999 5 ,while hydrochlorothiazide in the range of 51 .0-509 .6 μg ? mL - 1 ,r= 0 .999 8 .The average recovery of benazepril hydrochloride was between 99 .6% -101 .6% ,RSD < 2% .The average recovery of hydrochlorothiaz-ide was between 99 .3% -100 .7% ,RSD < 2% .Conclusion This method is simple ,accurate and reliable for the quality control of Benazepril Hydrochloride and Hydrochlorothiazide Tablets .