CAJ | 학술논문

目的：建立同时测定人血浆中异烟肼、吡嗪酰胺、利福平和左氧氟沙星的质量浓度的高效液相色谱法。方法血浆样品采用甲醇沉淀蛋白法萃取，采用 C18色谱柱（150 mm ×4．6 mm ，5．0μm），用甲醇，乙腈，0．05 mol ? L -1 KH2 PO3水溶液（三乙胺调pH6．0）进行梯度洗脱，柱温40℃，进样量20μL ，采用 PDA 紫外检测器。结果以峰面积外标法定量，异烟肼在0．20～25．6μg ? mL -1范围内与峰面积线性关系良好（r＝0．9997），吡嗪酰胺在0．20～25．6μg ? mL -1范围内与峰面积线性关系良好（r＝0．9999），利福平在0．40～25．6μg ? mL -1范围内与峰面积线性关系良好（r ＝0．9993），左氧氟沙星在0．20～25．6μg ? mL -1范围内与峰面积线性关系良好（r ＝0．9994），回收率为85％～115％，日内、日间精密度 RSD 均＜9．6％（n ＝5）。结论该方法专属性强、灵敏度高，准确性好，适用于同时测定人血浆样品中异烟肼、吡嗪酰胺、利福平和左氧氟沙星的质量浓度。
목적：건립동시측정인혈장중이연정、필진선알、리복평화좌양불사성적질량농도적고효액상색보법。방법혈장양품채용갑순침정단백법췌취，채용 C18색보주（150 mm ×4．6 mm ，5．0μm），용갑순，을정，0．05 mol ? L -1 KH2 PO3수용액（삼을알조pH6．0）진행제도세탈，주온40℃，진양량20μL ，채용 PDA 자외검측기。결과이봉면적외표법정량，이연정재0．20～25．6μg ? mL -1범위내여봉면적선성관계량호（r＝0．9997），필진선알재0．20～25．6μg ? mL -1범위내여봉면적선성관계량호（r＝0．9999），리복평재0．40～25．6μg ? mL -1범위내여봉면적선성관계량호（r ＝0．9993），좌양불사성재0．20～25．6μg ? mL -1범위내여봉면적선성관계량호（r ＝0．9994），회수솔위85％～115％，일내、일간정밀도 RSD 균＜9．6％（n ＝5）。결론해방법전속성강、령민도고，준학성호，괄용우동시측정인혈장양품중이연정、필진선알、리복평화좌양불사성적질량농도。
Objective To develop an HPLC method for the simultaneous determination of isoniazid ,pyrazinamide ,rifampicin and levofloxacin in human plasma .Methods The compounds were extracted from the plasma by a protein precipitation procedure with meth-anol .The LC separation of the analytes was performed on a C18 column (150 mm × 4 .6 mm ,5 .0 μm) at 40 ℃ ,using a gradient elution program with solvents consisting of methanol ,acetonitrile and 0 .05 mol ? L - 1 KH2 PO3-H2 O .The injection volume was 20 μL .The compounds were detected by PDA .Results Calibration curves in spiked plasma were linear in the range of 0 .20-25 .6 ,0 .20-25 .6 , 0 .40-25 .6 and 0 .20-25 .6 μg ? mL - 1 for isoniazid ,pyrazinamide ,rifampicin and levofloxacin ,respectively .The recoveries were between 85% -115% ,and the intra- and inter-day RSDs were < 9 .6% .Conclusion The method is specific ,sensitive and accurate ,and can be used for the simultaneous determination of isoniazid ,pyrazinamide ,rifampicin and levofloxacin in human plasma .