CAJ | 학술논문

目的：建立金线草中没食子酸含量测定方法。方法采用 HPLC 法，色谱柱以十八烷基硅烷键合硅胶为填充剂（250 mm ×4．6 mm ，5μm），甲醇-1 mL ? L -1磷酸溶液（4∶96）为流动相洗脱，检测波长272 nm ，体积流量1 mL ? min -1，柱温30℃。结果没食子酸在0．0410～0．2050μg 范围内（r ＝1）有良好的线性关系；平均回收率为101．3％，RSD 为1．7％（n ＝6）。结论该方法简便准确，为更好地开发利用金线草提供依据。
목적：건립금선초중몰식자산함량측정방법。방법채용 HPLC 법，색보주이십팔완기규완건합규효위전충제（250 mm ×4．6 mm ，5μm），갑순-1 mL ? L -1린산용액（4∶96）위류동상세탈，검측파장272 nm ，체적류량1 mL ? min -1，주온30℃。결과몰식자산재0．0410～0．2050μg 범위내（r ＝1）유량호적선성관계；평균회수솔위101．3％，RSD 위1．7％（n ＝6）。결론해방법간편준학，위경호지개발이용금선초제공의거。
Objective To establish an HPLC method for the determination of gallic acid in different parts of A ntenoron f ili f orme . Methods The test was carried out on a C18 column(250 mm × 4 .6 mm ,5 μm) ,with methanol 1 mL ? L - 1 phosphoric acid solution (4 ∶ 96) as the mobile phase .The flow rate was 1 mL ? min - 1 and the detection wavelength was 272 nm ;the column temperature was 30 ℃ .Results The linear range of gallic acid was 0 .041 0-0 .205 0 μg (r = 1) ,and the average recovery was 101 .3% with RSD 1 .65% (n= 6) .Conclusion The method is simple and accurate ,and provides a basis for the further development of Antenoron f iliforme .