CAJ | 학술논문

目的：采用石油醚、乙醇、氯仿、碱液4种溶剂提取紫草素，选出最优提取方法。方法紫草质量与提取溶剂体积比为1∶9，室温下搅拌浸提2次，用高效液相色谱梯度洗脱测定提取物中紫草素的含量，以紫草素提取率对提取方法进行评价。结果新疆紫草4种溶剂中紫草素含量高低顺序依次为碱液、乙醇、氯仿、石油醚提取物。结论碱液提取可提高提取物中紫草素的含量，并可减少有机溶剂的用量，经济、环保、实用。
목적：채용석유미、을순、록방、감액4충용제제취자초소，선출최우제취방법。방법자초질량여제취용제체적비위1∶9，실온하교반침제2차，용고효액상색보제도세탈측정제취물중자초소적함량，이자초소제취솔대제취방법진행평개。결과신강자초4충용제중자초소함량고저순서의차위감액、을순、록방、석유미제취물。결론감액제취가제고제취물중자초소적함량，병가감소유궤용제적용량，경제、배보、실용。
Objective To optimize the extraction of shikonin from A rnebia euchroma with solvent extraction in four solvents .Meth-ods The extractions were carried out in petroleum ether ,ethanol ,chloroform ,and 10 g ? L - 1 NaOH solution with stirring at 25 ℃ ,solvent extraction time of 30 min ,and solid to liquid ratio 1 ∶ 9 .The extraction process was conducted in duplicate .The shikonin content in extracts were measured by HPLC with gradient elution .Extraction methods were evaluated by extraction yield .Results The yield of shikonin extraction differed in four solvents (10 g ? L - 1 NaOH solution > ethanol > chloroform > petro-leum ether) .Conclusion The shikonin extraction in NaOH solution provides higher extraction yield and significantly reduces the amount of organic solvent .This economic and environmental method is suitable for shikonin extraction from A rnebia enchroma .