CAJ | 학술논문

目的：用紫外可见分光光度法测定苣荬菜根部总三萜皂苷的含量。方法采用AB-8、D-101、D-4020、HPD-400和NKA-95种大孔树脂对苣荬菜根部总三萜皂苷进行纯化，以齐墩果酸为对照品，50 g · L -1香草醛-冰醋酸、高氯酸为显色系统，在546 nm波长处测定总三萜皂苷含量。结果在AB-8、D-101、D-4020、HPD-400和NKA-95种树脂中，D-101对苣荬菜根部总三萜皂苷纯化效果最好；齐墩果酸质量浓度在0．0342～0．1065mg·mL -1范围内，吸光度与含量呈良好的线性关系，回归方程Y ＝8．2982 X-0．0849（r＝0．9996），平均加样回收率为95．3％，RSD＝1．5％（n＝5），苣荬菜根部总三萜皂苷含量为0．202％，RSD＝2．6％。结论该方法操作简单，快速灵敏，准确可靠，对设备要求不高，适用于苣荬菜根部总三萜皂苷的含量测定。
목적：용자외가견분광광도법측정거매채근부총삼첩조감적함량。방법채용AB-8、D-101、D-4020、HPD-400화NKA-95충대공수지대거매채근부총삼첩조감진행순화，이제돈과산위대조품，50 g · L -1향초철-빙작산、고록산위현색계통，재546 nm파장처측정총삼첩조감함량。결과재AB-8、D-101、D-4020、HPD-400화NKA-95충수지중，D-101대거매채근부총삼첩조감순화효과최호；제돈과산질량농도재0．0342～0．1065mg·mL -1범위내，흡광도여함량정량호적선성관계，회귀방정Y ＝8．2982 X-0．0849（r＝0．9996），평균가양회수솔위95．3％，RSD＝1．5％（n＝5），거매채근부총삼첩조감함량위0．202％，RSD＝2．6％。결론해방법조작간단，쾌속령민，준학가고，대설비요구불고，괄용우거매채근부총삼첩조감적함량측정。
Objective To establish a new method for the determination of total triterpenes from the roots of Sonchus arvensis L .by spectrophotometry .Methods By using AB-8 ,D-101 ,D-4020 ,HPD-400 and NKA-9 five kinds of macroporous resins for the pur-ification of total triterpenes from the roots of S .arvensis L .,with oleanolic acid as standard ,50 g · L -1 vanillin-acetic acid ,per-chloric acid as coloring systems ,triterpenes was measured by spectrophotometry and detected at 546 nm .Results D-101 macro-porous resin showed the best performance for the purification of the total triterpenes from the roots of S .arvensis L ..Absorbance and content of oleanolic acid showed a good linear relationship in the range of 0 .034 2-0 .106 5 mg · mL-1 .The regression equation was Y=8 .298 2X-0 .084 9(r=0 .999 6) ,and the average recovery rate was 95 .3% ,RSD=1 .5% (n=5) .The content of total triter-penes in the roots of S .arvensis L .was 0 .202% ,RSD = 2 .6% .Conclusion This method is simple ,fast and sensitive ,accurate and re-liable ,less demanding on the device ,and can be used for the content determination of total triterpenes from the roots of S .arvensis L ..