徐州医学院学报
徐州醫學院學報
서주의학원학보
ACTA ACADEMIAE MEDICINAE XUZHOU
2014年
9期
601-603
,共3页
单纯性热性惊厥%神经元特异性烯醇化酶%低氧诱导因子-1α%儿童
單純性熱性驚厥%神經元特異性烯醇化酶%低氧誘導因子-1α%兒童
단순성열성량궐%신경원특이성희순화매%저양유도인자-1α%인동
simple febrile convulsion%neuron specific enolase%hypoxia inducible factor -1α%children
目的：观察单纯性热性惊厥（simple febrile convulsion，SFC）患儿不同病程的神经元特异性烯醇化酶（ neuron specific enolase ，NSE）、低氧诱导因子-1α（ hypoxia inducible factor -1α，HIF-1α）的表达水平并探讨其意义，为SFC的临床诊治和预后判断提供科学依据。方法选择SFC患儿53例，分别于惊厥后24、72 h采取静脉血，另选健康对照组33例采血。采用酶联免疫吸附试验（ ELISA）测定血清NSE和HIF-1α水平。结果 SFC患儿NSE和HIF-1α水平明显高于对照组，差异有统计学意义（P＜0．05）；SFC患儿NSE和HIF-1α在惊厥后72 h水平高于24 h水平，但差异无统计学意义（ P＞0．05）。结论外周血NSE和HIF-1α水平的联合测定可作为SFC患儿脑损伤判断的新方法之一。
目的：觀察單純性熱性驚厥（simple febrile convulsion，SFC）患兒不同病程的神經元特異性烯醇化酶（ neuron specific enolase ，NSE）、低氧誘導因子-1α（ hypoxia inducible factor -1α，HIF-1α）的錶達水平併探討其意義，為SFC的臨床診治和預後判斷提供科學依據。方法選擇SFC患兒53例，分彆于驚厥後24、72 h採取靜脈血，另選健康對照組33例採血。採用酶聯免疫吸附試驗（ ELISA）測定血清NSE和HIF-1α水平。結果 SFC患兒NSE和HIF-1α水平明顯高于對照組，差異有統計學意義（P＜0．05）；SFC患兒NSE和HIF-1α在驚厥後72 h水平高于24 h水平，但差異無統計學意義（ P＞0．05）。結論外週血NSE和HIF-1α水平的聯閤測定可作為SFC患兒腦損傷判斷的新方法之一。
목적：관찰단순성열성량궐（simple febrile convulsion，SFC）환인불동병정적신경원특이성희순화매（ neuron specific enolase ，NSE）、저양유도인자-1α（ hypoxia inducible factor -1α，HIF-1α）적표체수평병탐토기의의，위SFC적림상진치화예후판단제공과학의거。방법선택SFC환인53례，분별우량궐후24、72 h채취정맥혈，령선건강대조조33례채혈。채용매련면역흡부시험（ ELISA）측정혈청NSE화HIF-1α수평。결과 SFC환인NSE화HIF-1α수평명현고우대조조，차이유통계학의의（P＜0．05）；SFC환인NSE화HIF-1α재량궐후72 h수평고우24 h수평，단차이무통계학의의（ P＞0．05）。결론외주혈NSE화HIF-1α수평적연합측정가작위SFC환인뇌손상판단적신방법지일。
Objective To observe the expression levels of neuron specific enolase ( NSE ) and hypoxia inducible factor-1α( HIF-1α) in children with simple febrile convulsion ( SFC) at different disease stages and to explore its clinical significance , so as to provide scientific basis for clinical diagnosis , treatment and judgment of prognosis for SFC . Methods Fifty-three cases of SFC were observed in this study .Venous blood samples were collected at 24 h and 72 h after convulsions .Blood samples from 33 healthy controls ( control group ) were also collected .Serum levels of NSE and HIF-1αwere detected by enzyme -linked immunosorbent assay ( ELISA) .Results NSE and HIF-1αlevels in chil-dren with SFC were significantly higher than that in the control group (P<0.05).NSE and HIF-1αlevels in children with SFC were higher at 72 h after the convulsion than at 24 h after the convulsion , but with no statistical significant difference (P>0.05).Conclusion Combined determination of peripheral blood NSE and HIF -1αlevels can be one of the new detection method for judging the SFC in children with brain damage .
