CAJ | 학술논문

目的：探讨大鼠骨髓间充质干细胞条件培养基（ BMSCs-CM）对2型糖尿病大鼠脑缺血/再灌注损伤后缺血半暗带区血管新生的影响。方法取雄性Wistar大鼠股骨骨髓作为供体来源制备BMSCs-CM。链脲佐菌素-烟酰胺诱导2型糖尿病大鼠模型，成功后用线栓法制备大脑中动脉缺血/再灌注损伤模型，缺血2h后血管再通。随机分为生理盐水对照组（ NS组）、单纯培养基对照组（ DMEM组）、骨髓间充质干细胞条件培养基组（CM组）。术后2、24、48 h，CM组大鼠经尾静脉给予BMSCs-CM（10 ml/kg），DMEM组给予DMEM（10 ml/kg）， NS组给予生理盐水（10 ml/kg）。术后14天，分别比较3组大鼠脑缺血半暗带区血管性血友病因子（ vWF）和血管生成素-1（ Ang1）的变化情况。结果术后14天，CM组与其他2组相比，脑缺血半暗带区vWF阳性血管密度及阳性血管周长均明显增加，差异有统计学意义（P＜0．05），而NS组、DMEM组相比，差异无统计学意义（P＞0．05）；CM组与其他2组相比，大鼠脑缺血半暗带区Ang1表达量显著增加，差异有统计学意义（P＜0．01），而NS组、DMEM组相比，差异无统计学意义（P＞0．05）。结论大鼠BMSCs-CM可促进2型糖尿病大鼠脑缺血/再灌注损伤后缺血半暗带区血管新生。
목적：탐토대서골수간충질간세포조건배양기（ BMSCs-CM）대2형당뇨병대서뇌결혈/재관주손상후결혈반암대구혈관신생적영향。방법취웅성Wistar대서고골골수작위공체래원제비BMSCs-CM。련뇨좌균소-연선알유도2형당뇨병대서모형，성공후용선전법제비대뇌중동맥결혈/재관주손상모형，결혈2h후혈관재통。수궤분위생리염수대조조（ NS조）、단순배양기대조조（ DMEM조）、골수간충질간세포조건배양기조（CM조）。술후2、24、48 h，CM조대서경미정맥급여BMSCs-CM（10 ml/kg），DMEM조급여DMEM（10 ml/kg）， NS조급여생리염수（10 ml/kg）。술후14천，분별비교3조대서뇌결혈반암대구혈관성혈우병인자（ vWF）화혈관생성소-1（ Ang1）적변화정황。결과술후14천，CM조여기타2조상비，뇌결혈반암대구vWF양성혈관밀도급양성혈관주장균명현증가，차이유통계학의의（P＜0．05），이NS조、DMEM조상비，차이무통계학의의（P＞0．05）；CM조여기타2조상비，대서뇌결혈반암대구Ang1표체량현저증가，차이유통계학의의（P＜0．01），이NS조、DMEM조상비，차이무통계학의의（P＞0．05）。결론대서BMSCs-CM가촉진2형당뇨병대서뇌결혈/재관주손상후결혈반암대구혈관신생。
Objective To investigate the effects of bone mesenchymal stem cell conditioned medium ( BMSC-CM) on the angiogenesis of type 2 diabetic (T2DM) rats after ischemia/reperfusion.Methods BMSC-CM was prepared u-sing the bone marrow from the fermur of male wistar rats .A model of type 2 diabetic rats was established by injection of streptozotocin-nicotinamide.Then the obtained T2DM rats were subjected to middle cerebral artery occlusion (MCAo) using a nylon monofilament suture followed by reperfusion 2 hours later.After surgery, the T2DM ischemic rats were ran-domly assigned to three groups according to intravenous injection of 10 ml/kg normal saline ( NS) , 10 ml/kg DMEM cul-ture medium or 10 ml/kg BMSC-CM through their tail vein 2, 24 and 48 hours after surgery.On day 14, the changes of von Willebrand factor (vWF) and angiopoietin 1 (Ang1) in the ischemic penumbra were detected by immunostaining . Results Compared to the NS and DMEM groups , the CM group presented significant increases in vWF positive vascular density and vascular perimeter (P<0.05).However, there was no significant difference between the NS and DMEM groups (P>0.05).Compared to the NS and DMEM groups, remarkably increased amounts of Ang1 were detected in the CM group (P<0.01).However, there was no significant difference between the NS and DMEM groups as to Ang 1 ex-pression (P>0.05).Conclusion Bone mesenchymal stem cell conditioned medium can stimulate the angiogenesis within the ischemic penumbra of T2DM rats after ischemia/reperfusion.