CAJ | 학술논문

目的：探讨人胰腺癌组织中Livin与血管内皮生长因子（VEGF）的表达情况和临床意义。方法采用免疫组织化学法检测68例胰腺癌组织中Livin及VEGF蛋白的表达，并以44例癌旁组织作为对照。结果 Livin在胰腺癌组织及癌旁组织中的阳性表达率分别为73．5％和4．5％；Livin在44例癌组织及配对癌旁组织中的阳性表达率比较，差异有统计学意义（χ2＝48．137，P＜0．001）；VEGF在胰腺癌组织及癌旁组织中的阳性表达率分别为69．1％和13．6％；VEGF在44例癌组织及配对癌旁组织中的阳性表达率比较，差异有统计学意义（χ2＝29．147，P＜0．001）。Livin及VEGF蛋白的表达均与肿瘤分化程度（χ2＝6．061，P＝0．014；χ2＝6．592，P＝0．010）、临床TNM分期（χ2＝4．175，P＝0．041；χ2＝9．992， P＝0．002）、区域淋巴结转移（χ2＝11．731，P＝0．001；χ2＝12．002，P＝0．001）及神经浸润（χ2＝9．950， P＝0．002；χ2＝7．433，P＝0．006）有关。Spearman相关性分析表明Livin与VEGF在胰腺癌组织中的阳性表达呈正相关（r＝0．320，P＝0．008）。生存分析表明，Livin及VEGF 均是胰腺癌预后的独立因素。结论 Livin及VEGF参与了胰腺癌的发展、侵袭和转移，推测Livin可能通过上调VEGF促进肿瘤血管生成和肿瘤细胞迁移。
목적：탐토인이선암조직중Livin여혈관내피생장인자（VEGF）적표체정황화림상의의。방법채용면역조직화학법검측68례이선암조직중Livin급VEGF단백적표체，병이44례암방조직작위대조。결과 Livin재이선암조직급암방조직중적양성표체솔분별위73．5％화4．5％；Livin재44례암조직급배대암방조직중적양성표체솔비교，차이유통계학의의（χ2＝48．137，P＜0．001）；VEGF재이선암조직급암방조직중적양성표체솔분별위69．1％화13．6％；VEGF재44례암조직급배대암방조직중적양성표체솔비교，차이유통계학의의（χ2＝29．147，P＜0．001）。Livin급VEGF단백적표체균여종류분화정도（χ2＝6．061，P＝0．014；χ2＝6．592，P＝0．010）、림상TNM분기（χ2＝4．175，P＝0．041；χ2＝9．992， P＝0．002）、구역림파결전이（χ2＝11．731，P＝0．001；χ2＝12．002，P＝0．001）급신경침윤（χ2＝9．950， P＝0．002；χ2＝7．433，P＝0．006）유관。Spearman상관성분석표명Livin여VEGF재이선암조직중적양성표체정정상관（r＝0．320，P＝0．008）。생존분석표명，Livin급VEGF 균시이선암예후적독립인소。결론 Livin급VEGF삼여료이선암적발전、침습화전이，추측Livin가능통과상조VEGF촉진종류혈관생성화종류세포천이。
Objective To investigate the expressions of Livin and vascular endothelial growth factor (VEGF)in pancreatic carcinoma and their cilinical significances.Methods The expressions of Livin and VEGF proteins were tested by immunohistochemistry in 68 cases of pancreatic carcinomas and 44 cases of adja-cent paracancerous tissues.Results The positive rates of Livin in pancreatic carcinomas and adjacent paracan-cerous tissues were 73.5% and 4.5% respectively,and the difference was statistically significant (χ2 =48.137,P<0.001).The positive rates of VEGF in pancreatic carcinomas and adjacent paracancerous tissues were 69.1% and 13.6% respectively,and the difference was statistically significant (χ2 =29.147,P <0.001).The expressions of Livin and VEGF were related with tumor differentiation (χ2 =6.061,P=0.014;χ2 =6.592,P=0.010),TNMstage (χ2 =4.175,P=0.041;χ2 =9.992,P=0.002),lymph node metasta-sis (χ2 =11.731,P=0.001;χ2 =12.002,P=0.001)and neural invasion (χ2 =9.950,P=0.002;χ2 =7.433,P=0.006).Significantly positive correlation was found between the expressions of Livin and VEGF by using Spearman correlation analysis (r=0.320,P=0.008).Survival analysis showed that the expressions of Livin and VEGF were independent prognostic factors in pancreatic carcinoma.Conclusion Livin and VEGF involve in the development,migration and metastasis of pancreatic carcinoma.Livin may upregulate the expres-sion of VEGF,which may lead to the angiogenesis and migration in pancreatic carcinoma.