CAJ | 학술논문

目的：检测血浆中microRNA（miR）-155-5p、miR-21-5p、miR-29a、miR-142-5p在健康对照组、活动性肺结核组以及潜伏结核感染组中的表达情况，探讨其作为诊断结核病生物标志的可能性。方法健康对照组60人、活动性肺结核组40人和潜伏结核感染组20人，利用反转录-荧光定量PCR检测其血浆中miR-155-5p、miR-21-5p、miR-29a、miR-142-5p的表达水平。结果与健康对照组相比，活动性肺结核组血浆中除miR-142-5p外，miR-155-5p、miR-21-5p和miR-29a都出现表达上调的现象，分别是健康对照组的10．13倍、7．34倍和2．74倍，差异有统计学意义（P＜0．05）；ROC曲线分析miR-155-5p、miR-21-5p和miR-29a，曲线下面积（AUC）分别为0．905、0．830和0．687。与健康对照组相比，潜伏结核感染组血浆中只有miR-155-5p表达上调，且是健康对照者的3．1倍，差异有统计学意义（P＜0．05），miR-21-5p、miR-29a和miR-142-5p表达无差异。与潜伏结核感染组相比，活动性肺结核组血浆中除miR-142-5p外，miR-155-5p、miR-21-5p和miR-29a均表达上调，分别是潜伏结核感染组的3．26倍、6．69倍和1．98倍，差异有统计学意义（ P＜0．05）。与活动性肺结核患者密切接触者中潜伏结核感染率为40．58％。结论 miR-155-5p、miR-21-5p和miR-29a在健康对照组和活动性肺结核组以及活动性肺结核组和潜伏结核感染组之间存在差异性表达，miR-142-5p在3组之间均无差异性表达。 miR-155-5p、miR-21-5p和miR-29a可能是潜在诊断活动性肺结核、潜伏结核感染的生物标志物。与活动性肺结核患者密切接触者中潜伏结核感染率较高。
목적：검측혈장중microRNA（miR）-155-5p、miR-21-5p、miR-29a、miR-142-5p재건강대조조、활동성폐결핵조이급잠복결핵감염조중적표체정황，탐토기작위진단결핵병생물표지적가능성。방법건강대조조60인、활동성폐결핵조40인화잠복결핵감염조20인，이용반전록-형광정량PCR검측기혈장중miR-155-5p、miR-21-5p、miR-29a、miR-142-5p적표체수평。결과여건강대조조상비，활동성폐결핵조혈장중제miR-142-5p외，miR-155-5p、miR-21-5p화miR-29a도출현표체상조적현상，분별시건강대조조적10．13배、7．34배화2．74배，차이유통계학의의（P＜0．05）；ROC곡선분석miR-155-5p、miR-21-5p화miR-29a，곡선하면적（AUC）분별위0．905、0．830화0．687。여건강대조조상비，잠복결핵감염조혈장중지유miR-155-5p표체상조，차시건강대조자적3．1배，차이유통계학의의（P＜0．05），miR-21-5p、miR-29a화miR-142-5p표체무차이。여잠복결핵감염조상비，활동성폐결핵조혈장중제miR-142-5p외，miR-155-5p、miR-21-5p화miR-29a균표체상조，분별시잠복결핵감염조적3．26배、6．69배화1．98배，차이유통계학의의（ P＜0．05）。여활동성폐결핵환자밀절접촉자중잠복결핵감염솔위40．58％。결론 miR-155-5p、miR-21-5p화miR-29a재건강대조조화활동성폐결핵조이급활동성폐결핵조화잠복결핵감염조지간존재차이성표체，miR-142-5p재3조지간균무차이성표체。 miR-155-5p、miR-21-5p화miR-29a가능시잠재진단활동성폐결핵、잠복결핵감염적생물표지물。여활동성폐결핵환자밀절접촉자중잠복결핵감염솔교고。
Objective To evaluate the possibilities of using circulating miR-155-5p, miR-21-5p, miR-29a and miR-142-5p as biomarkers for the diagnosis of active pulmonary tuberculosis (TB).Methods Plasma samples were collected from 60 healthy subjects, 40 patients with active pulmonary TB and 20 sub-jects with latent tuberculosis infection ( LTBI) to extract miRNAs.The levels of miR-155-5p, miR-21-5p, miR-29a and miR-142-5p in plasma samples were detected by using reverse transcription-quantitative PCR. Results The levels of miR-155-5p, miR-21-5p and miR-29a in patients with active pulmonary TB were re-spectively 10.13, 7.34 and 2.74 times as much as those in healthy subjects(P<0.05).No significant differences with the level of miR-142-5p were observed between the two groups.The receiver operating char-acteristic (ROC) curve analysis of miR-155-5p, miR-21-5p and miR-29a in patients with active pulmonary TB and healthy subjects showed that the areas under the curve (AUC) were respectively 0.905, 0.830 and 0.687.The level of miR-155-5p in patients with LTBI was 3.1 times than that in healthy subjects ( P<0. 05).No differences with miR-21-5p, miR-29a and miR-142-5p were found between patients with LTBI and healthy subjects.The levels of miR-155-5p, miR-21-5p and miR-29a in patients with active pulmonary TB were respectively 3.26, 6.69 and 1.98 times than those in patients with LTBI (P<0.05).The rate of LTBI was 40.58%in people who were in close contact with patients with active pulmonary TB.Conclusion Sig-nificant differences with the levels of miR-155-5p, miR-21-5p and miR-29a were observed among healthy subjects, patients with active pulmonary TB and patients with LTBI, but no difference with the level of miR-142-5p was observed among them.miR-155-5p, miR-21-5p and miR-29a could be used as the potential bio-markers for the diagnosis of active pulmonary tuberculosis and latent tuberculosis infection.People who were in close contact with patients with active pulmonary TB could have a higher LTBI rate.