CAJ | 학술논문

目的：了解深圳地区柯萨奇病毒A2型（ CVA2） SHZH13-01株的基因特征，研究CVA2的进化及变异状况。方法应用RT-PCR法扩增SHZH13-01株全基因组，PCR产物纯化后进行序列测定和基因进化分析。结果 CVA2-SHZH13-01株的全基因组由7400个核苷酸组成，编码2191个氨基酸。病毒的5′非编码区（UTR）、P1（VP1～VP4）、P2、P3、3′非编码区和全基因组的核苷酸与近年香港分离的新型重组CVA2-HK（431306）序列同源性最高，分别为98．3％、98．8％、99．0％、99．2％、98．8％，98．9％。而与其他肠道病毒相比，SHZH13-01在结构蛋白P1区与CVA2国际原型株Fleet-wood同源性最高，达81．6％，而在非结构蛋白区P2、P3与EV71病毒来自同一分支，同源性达82．8％～88．7％。根据系统进化分析，深圳SHZH13-01病毒株属于CVA2-HK（431306）病毒变异株。通过与CVA2-HK（431306）衣壳区氨基酸比对，CVA2-SHZH13-01的变异位点变化情况为： aa5L→F， aa666S→G， aa671T→I。结论柯萨奇病毒A2型深圳株SHZH13-01属于香港新型重组CVA2-HK（431306株）的变异株。
목적：료해심수지구가살기병독A2형（ CVA2） SHZH13-01주적기인특정，연구CVA2적진화급변이상황。방법응용RT-PCR법확증SHZH13-01주전기인조，PCR산물순화후진행서렬측정화기인진화분석。결과 CVA2-SHZH13-01주적전기인조유7400개핵감산조성，편마2191개안기산。병독적5′비편마구（UTR）、P1（VP1～VP4）、P2、P3、3′비편마구화전기인조적핵감산여근년향항분리적신형중조CVA2-HK（431306）서렬동원성최고，분별위98．3％、98．8％、99．0％、99．2％、98．8％，98．9％。이여기타장도병독상비，SHZH13-01재결구단백P1구여CVA2국제원형주Fleet-wood동원성최고，체81．6％，이재비결구단백구P2、P3여EV71병독래자동일분지，동원성체82．8％～88．7％。근거계통진화분석，심수SHZH13-01병독주속우CVA2-HK（431306）병독변이주。통과여CVA2-HK（431306）의각구안기산비대，CVA2-SHZH13-01적변이위점변화정황위： aa5L→F， aa666S→G， aa671T→I。결론가살기병독A2형심수주SHZH13-01속우향항신형중조CVA2-HK（431306주）적변이주。
Objective To analyze the complete genome sequence of a Shenzhen coxsackievirus A2 strain CVA2-SHZH13-01 and its evolution.Methods RT-PCR was used to amplify the complete genome of CVA2-SHZH13-01 strain.The PCR products were purified and sequenced to analyze their genetic character-istics.Results The complete genome of CVA2-SHZH13-01 strain was 7400 bp in length, encoding 2191 amino acids.CVA2-SHZH13-01 strain was highly similar with the novel recombinant CVA2-HK (431306) strain isolated from Hong Kong sharing the nucleotide homology of 98.3%, 98.8%, 99.0%, 99.2%, 98.8%and 98.9%in 5′UTR, P1 ( VP1 to VP4) , P2, P3, 3′UTR regions and complete genome, respec-tively.CVA2-SHZH13-01 strain was highly identical to the international standard strain CVA2-Fleetwood showing the homology of 81.6% in nucleotide sequences in P1 region, but closely associated with EV71-SHZH03 and EV71-GD2009 strains (82.8%-88.7%) in P2 and P3 regions.The phylogenetic analysis in-dicated that CVA2-SHZH13-01 strain belonged to the CVA2-HK (431306) variant.Data from analysis of amino acid in P1 region showed that there were three amino acid mutations in CVA2-SHZH13-01 strain including aa5L→F, aa666S→G and aa671T→I as compared with CVA2-HK (431306) strain.Conclusion CVA2-SHZH13-01 strain belonged to CVA2-HK (431306) variant.