CAJ | 학술논문

目的：初步了解深圳市呼吸道合胞病毒的流行规律及分子变异特点。方法荧光RT-PCR对流感样病例样本进行初筛后，通过巢式RT-PCR对病毒G蛋白基因C端的可变区进行扩增，利用MEGA等软件进行分子变异分析。结果呼吸道合胞病毒在深圳的流行较为普遍，流行高峰一般出现在春季和夏秋季；流行的病毒有A、B两个亚型， NA1和BAⅨ分别是其流行株系的基因型；病毒G蛋白C端的变异位点较为散乱，少数变异导致了病毒糖基化位点消失。一株包含24个氨基酸插入序列的新型重组毒株出现在2013年，此重组株很有可能是由国外传入我国。结论呼吸道合胞病毒在深圳存在持续而广泛的流行，而且病毒仍处在不断的进化和变异之中。
목적：초보료해심수시호흡도합포병독적류행규률급분자변이특점。방법형광RT-PCR대류감양병례양본진행초사후，통과소식RT-PCR대병독G단백기인C단적가변구진행확증，이용MEGA등연건진행분자변이분석。결과호흡도합포병독재심수적류행교위보편，류행고봉일반출현재춘계화하추계；류행적병독유A、B량개아형， NA1화BAⅨ분별시기류행주계적기인형；병독G단백C단적변이위점교위산란，소수변이도치료병독당기화위점소실。일주포함24개안기산삽입서렬적신형중조독주출현재2013년，차중조주흔유가능시유국외전입아국。결론호흡도합포병독재심수존재지속이엄범적류행，이차병독잉처재불단적진화화변이지중。
Objective To study the epidemic pattern and molecular variation of respiratory syncy-tial virus ( RSV) strains isolated in Shenzhen from year 2012 to 2013.Methods The clinical samples iso-lated from patients with influenza-like illness were analyzed by fluorescent RT-PCR to screen RSV positive strains.The C-terminal variable regions of genes encoding G proteins were amplified by nested RT-PCR. Molecular variation was analyzed by using Clustal W and MEGA softwares.Results RSV strains were wide-ly prevalent in Shenzhen from 2012 to 2013.Two epidemic peaks usually occurred in spring and summer/au-tumn of each year.The RSV isolates were subtyped into group A belonging to genotype NA1 and group B be-longing to genotype BAⅨ.Most of the mutations scattered at the C-terminal region of G protein.A few mu-tations caused the disappearance of certain glycosylation sites.A novel recombinant virus strain containing 24 inserted amino acids was identified in 2013, which was likely to be introduced into our country from abroad. Conclusion RSV strains were widely and continuously prevalent in Shenzhen, characterized by constant evolution and variation.