CAJ | 학술논문

目的：探讨子宫腺肌病患者外周血白介素-10（IL-10）、干扰素-γ（IFN-γ）水平在治疗前后的变化及其临床意义。方法选取55例子宫腺肌病患者，以30健康体检女性为对照，采用酶联免疫法（ELISA）测定外周血 IL-10及IFN-γ的含量，并根据子宫内膜入侵子宫肌层的不同程度对患者进行病理分级，观察外周血 IL-10及 IFN-γ水平在病例组与对照组、治疗前后及不同病理分级之间的差异。结果与对照组相比，子宫腺肌病患者血清 IL-10水平明显升高（t＝5．748，P＝0．000），IFN-γ明显降低（t＝3．217，P＝0．002）；手术治疗后，患者 IL-10水平明显下降（t＝2．957，P＝0．004），IFN-γ明显上升（t＝2．694，P＝0．009）；血清 IL-10水平在不同病理分级患者间具有显著差异，病理分级越高，IL-10水平越高，但血清 IFN-γ水平在不同病理分级患者间无显著差异（均P＞0．05）。结论子宫腺肌病患者血清IL-10及 IFN-γ水平明显异常，且 IL-10含量与疾病的病理分级明显相关，推测细胞因子紊乱在子宫腺肌病的发病机制和疾病进展中可能扮演重要角色。
목적：탐토자궁선기병환자외주혈백개소-10（IL-10）、간우소-γ（IFN-γ）수평재치료전후적변화급기림상의의。방법선취55례자궁선기병환자，이30건강체검녀성위대조，채용매련면역법（ELISA）측정외주혈 IL-10급IFN-γ적함량，병근거자궁내막입침자궁기층적불동정도대환자진행병리분급，관찰외주혈 IL-10급 IFN-γ수평재병례조여대조조、치료전후급불동병리분급지간적차이。결과여대조조상비，자궁선기병환자혈청 IL-10수평명현승고（t＝5．748，P＝0．000），IFN-γ명현강저（t＝3．217，P＝0．002）；수술치료후，환자 IL-10수평명현하강（t＝2．957，P＝0．004），IFN-γ명현상승（t＝2．694，P＝0．009）；혈청 IL-10수평재불동병리분급환자간구유현저차이，병리분급월고，IL-10수평월고，단혈청 IFN-γ수평재불동병리분급환자간무현저차이（균P＞0．05）。결론자궁선기병환자혈청IL-10급 IFN-γ수평명현이상，차 IL-10함량여질병적병리분급명현상관，추측세포인자문란재자궁선기병적발병궤제화질병진전중가능분연중요각색。
Objective To determinate the serum level of IL-10 and IFN-γbefore and after treatment in adenomyosis patients and its clinical significance.Methods A total of 55 patients with adenomyosis and 30 healthy controls were en-rolled in this study,serum IL-10 and IFN-γwere detected by enzyme linked immunosorbent assay (ELISA)and Patho-logical grading was determined according by different levels of myometrial invasion of endometrium.Statistical signifi-cance was compared between patients and healthy controls,before and after therapy,patients in different pathological grading.Results Compared with healthy controls,patients with adenomyosis had higher level of serum IL-10 (t=5.748,P=0.000)and lower level of serum IFN-γ(t=3.217,P=0.002);The level of serum IL-10 was down-regulated (t=2.957,P=0.004)and IFN-γwas up-regulated(t=2.694,P=0.009)after surgery;The level of serum IL-10 showed significant difference according to the patological grading:patients with higher pathological grade had higher se-rum IL-10 ,but the level of serum IFN-γdidn’t show any significant difference(all P>0.05).Conclusion There are significantly abnormal serum IL-10 and IFN-γin patients with adenomyosis and the serum level is correlated with the pathological grading,so it can be speculated that the disturbance of cytokines may play an important role in the patho-genesis of adenomyosis.