中国实验诊断学
中國實驗診斷學
중국실험진단학
CHINESE JOURNAL OF LABORATORY DIAGNOSIS
2014年
10期
1649-1651
,共3页
哮喘%IL-17F基因%突变%DNA序列分析
哮喘%IL-17F基因%突變%DNA序列分析
효천%IL-17F기인%돌변%DNA서렬분석
asthma%IL-17F gene%mutation%DNA sequencing
目的：探讨白细胞介素-17F基因多态性与儿童支气管哮喘的相关性，为支气管哮喘的易感基因筛选提供可能的理论依据。方法取本院门诊随诊的60例哮喘患儿设为哮喘组，60例健康儿童设为对照组，两组儿童均于清晨空腹留取外周静脉血2 ml，提取全基因组 DNA，应用聚合酶链反应及克隆、基因测序方法分析 IL-17F基因序列rs763780（H161R）基因多态性。结果 IL-17F基因序列 rs763780（H161R）基因型在两组内均未检测到突变型 CC， rs763780（H161R）基因型（TC/TT）在两组间内的分布有明显的差异（χ2＝5．71，P＜0．05）。TC基因型 OR和95％可信区间（CI）为2．09（1．12-3．90），TT基因型 OR和95％CI为0．76（0．60-0．95）。结论 IL-17F基因多态性 rs763780（H 161 R）基因型分布（TC/TT）在两组间存在差异，其多态性可能与哮喘相关，两组内均未发现基因型 CC在，基因型TC可能是哮喘发病的危险因素。
目的：探討白細胞介素-17F基因多態性與兒童支氣管哮喘的相關性，為支氣管哮喘的易感基因篩選提供可能的理論依據。方法取本院門診隨診的60例哮喘患兒設為哮喘組，60例健康兒童設為對照組，兩組兒童均于清晨空腹留取外週靜脈血2 ml，提取全基因組 DNA，應用聚閤酶鏈反應及剋隆、基因測序方法分析 IL-17F基因序列rs763780（H161R）基因多態性。結果 IL-17F基因序列 rs763780（H161R）基因型在兩組內均未檢測到突變型 CC， rs763780（H161R）基因型（TC/TT）在兩組間內的分佈有明顯的差異（χ2＝5．71，P＜0．05）。TC基因型 OR和95％可信區間（CI）為2．09（1．12-3．90），TT基因型 OR和95％CI為0．76（0．60-0．95）。結論 IL-17F基因多態性 rs763780（H 161 R）基因型分佈（TC/TT）在兩組間存在差異，其多態性可能與哮喘相關，兩組內均未髮現基因型 CC在，基因型TC可能是哮喘髮病的危險因素。
목적：탐토백세포개소-17F기인다태성여인동지기관효천적상관성，위지기관효천적역감기인사선제공가능적이론의거。방법취본원문진수진적60례효천환인설위효천조，60례건강인동설위대조조，량조인동균우청신공복류취외주정맥혈2 ml，제취전기인조 DNA，응용취합매련반응급극륭、기인측서방법분석 IL-17F기인서렬rs763780（H161R）기인다태성。결과 IL-17F기인서렬 rs763780（H161R）기인형재량조내균미검측도돌변형 CC， rs763780（H161R）기인형（TC/TT）재량조간내적분포유명현적차이（χ2＝5．71，P＜0．05）。TC기인형 OR화95％가신구간（CI）위2．09（1．12-3．90），TT기인형 OR화95％CI위0．76（0．60-0．95）。결론 IL-17F기인다태성 rs763780（H 161 R）기인형분포（TC/TT）재량조간존재차이，기다태성가능여효천상관，량조내균미발현기인형 CC재，기인형TC가능시효천발병적위험인소。
Objective To explore the correlation between IL-17F gene polymorphism and children asthma,providing some theoretical basis for pathophysiology and identification of susceptibility to asthma.Methods 60 Follow-up chil-dren with asthma in clinic were enrolled randomly to be asthma group,sixty healthy children were set as control group. 2 ml peripheral vein blood was drawn from each child with empty stomach in early morning.Each blood sample was ex-tracted genomic DNA,Polymerase chain reaction (PCR),gene clone and DNA sequencing were used to analyse the IL-17F polymorphisms (rs763780).Results The H161R homozygote (CC )is not found in both asthma group and health group.Significant deviations from the genotypes (TC/TT)of rs763780 were found in the asthma and health group.(χ2=5.71 P=0.017<0.05).The OR for asthma was 2.09 (95% CI,1.12-3.90)for the H161R mutant type(TC),the OR for asthma was 0.76 (95% CI,0.60-0.95)for the H161R wild type(TT).Conclusion Significant deviations from the genotypes (TC/TT)of IL-17F rs763780 were found between the asthma and control groups,the polymorphisms may be associated with asthma.The H161R homozygote (CC)is not found in both groups,The H161R mutant type (TC)may be the risk of asthma.
