CAJ | 학술논문

针对结核分枝杆菌保守靶基因IS6110，设计环介导等温扩增法（LAMP）特异性引物，实验结果表明，在65?C恒温条件下反应50分钟即可完成反应；在反应液中加入钙黄绿素后可通过颜色变化来判断反应结果，该LAMP方法的最低检出浓度为101pg/μl，与PCR方法的最低检出浓度1.01ng/μl相比提高了10倍，且具有良好的特异性。所以LAMP方法检测结核分枝杆菌具有简单、快速、价廉、特异性强、灵敏度高等特点，适用于疾控工作及临床筛查或快速检测结核杆菌。
침대결핵분지간균보수파기인IS6110，설계배개도등온확증법（LAMP）특이성인물，실험결과표명，재65?C항온조건하반응50분종즉가완성반응；재반응액중가입개황록소후가통과안색변화래판단반응결과，해LAMP방법적최저검출농도위101pg/μl，여PCR방법적최저검출농도1.01ng/μl상비제고료10배，차구유량호적특이성。소이LAMP방법검측결핵분지간균구유간단、쾌속、개렴、특이성강、령민도고등특점，괄용우질공공작급림상사사혹쾌속검측결핵간균。
Based on conservative target gene IS6110 of Mycobacterium tuberculosis, and designed a speciifcity LAMP primer set, the results showed that the LAMP assay of Mycobacterium tuberculosis can react at 65?C in 50 minutes, if we add some calcein in reaction buffer, we can judge the results by color change after the reaction. The limit of the LAMP assay is 101pg/μL of DNA concentration, while the limit of the PCR is 1.01ng/μl, the LAMP assay has 10 times higher sensitivity than the PCR, and with high speciifcity. So the LAMP assay has the advantages of simple, fast, cheap, high speciifcity and sensitivity, it suitable for Disease Control and prevention and clinical screening or rapid detection of Mycobacterium tuberculosis.