CAJ | 학술논문

目的：观察P53凋亡刺激蛋白（ASPP家族的抑制因子 iASPP）在压力诱导的视网膜神经节细胞（RGC-5）凋亡中的表达及意义。方法将视网膜神经节细胞（RGC-5细胞）分别在60mmHg和80mmHg压力条件下培养48小时，MTT方法检测细胞活力，细胞染色检测凋亡，RT-PCR，WESTERN-BLOT检测 P53和 iASPP基因的变化。结果随着压力的增加 RGC-5细胞活力下降，细胞凋亡逐渐增加，P53基因表达增加，iASPP基因的表达下降。结论在压力诱导的RGC-5细胞凋亡中iASPP 表达下降可能使P53活性增加，促进RGC-5细胞凋亡的发生，上调iASPP表达可能具有神经保护作用。
목적：관찰P53조망자격단백（ASPP가족적억제인자 iASPP）재압력유도적시망막신경절세포（RGC-5）조망중적표체급의의。방법장시망막신경절세포（RGC-5세포）분별재60mmHg화80mmHg압력조건하배양48소시，MTT방법검측세포활력，세포염색검측조망，RT-PCR，WESTERN-BLOT검측 P53화 iASPP기인적변화。결과수착압력적증가 RGC-5세포활력하강，세포조망축점증가，P53기인표체증가，iASPP기인적표체하강。결론재압력유도적RGC-5세포조망중iASPP 표체하강가능사P53활성증가，촉진RGC-5세포조망적발생，상조iASPP표체가능구유신경보호작용。
Objective This study was to explore the expression of iASPP on pressure-induced apoptosis of cultured retinal ganglion cells (RGC-5 cells).Methods The RGC-5 cells were cultured under the pressure of 60mmHg and 80mmHg for 48 hours.Cell viability were determined by MTT assays.Cells apoptosis was determined by Hoe33342. The expression of iASPP and P53 was determined by using semi-quantitative RT-PCR and Western blot respective. Results MTT assay showed that the RGC-5 cells viability was decreased in a pressure-dependent manner.As the pressure was up,the apoptosis of RGC-5 cells was increased,the expression of P53 was elevated and the expression of iASPP was decreased.Conclusion The reduced expression of iASPP gene may increase the activity of p53 and promote the apoptosis of RGC-5 cells under pressure.Enhancing expression of iASPP may play a role as a neuroprotect.