CAJ | 학술논문

目的：建立大鼠左颈总动脉与左侧颈外静脉吻合分流性肺动脉高压模型。方法成年雄性 Wistar大鼠随机分为三组：吻合组（A组，n＝15），结扎组（B组，n＝15），正常组（C组，n＝15）。饲养3月后测定血流动力学指标，右心室/左心室＋室间隔质量比，肺组织切片病理变化，ELISA方法测定血浆 ET-1浓度，荧光定量 PCR测定肺组织表达内皮素-1 mRNA的变化。结果与B，C两组相比，A组：右心室收缩压力明显升高，血清内皮素-1水平升高，荧光定量PCR结果显示 A组内皮素-1mRNA的相对表达量是B组的2．601倍，是 C组的3．373倍，B组是 C组的1．297（P＜0．05）。结论通过分离左颈总动脉及左颈外静脉切断后，近心端吻合，可成功建立模拟先天性心脏病的分流性肺动脉高压模型，作为肺动脉高压标志的内皮素-1在此模型中处于高表达状态。
목적：건립대서좌경총동맥여좌측경외정맥문합분류성폐동맥고압모형。방법성년웅성 Wistar대서수궤분위삼조：문합조（A조，n＝15），결찰조（B조，n＝15），정상조（C조，n＝15）。사양3월후측정혈류동역학지표，우심실/좌심실＋실간격질량비，폐조직절편병리변화，ELISA방법측정혈장 ET-1농도，형광정량 PCR측정폐조직표체내피소-1 mRNA적변화。결과여B，C량조상비，A조：우심실수축압력명현승고，혈청내피소-1수평승고，형광정량PCR결과현시 A조내피소-1mRNA적상대표체량시B조적2．601배，시 C조적3．373배，B조시 C조적1．297（P＜0．05）。결론통과분리좌경총동맥급좌경외정맥절단후，근심단문합，가성공건립모의선천성심장병적분류성폐동맥고압모형，작위폐동맥고압표지적내피소-1재차모형중처우고표체상태。
Objective To establish arteriovenous shunt of pulmonary artery hypertension by anastomosis of the left common carotid artery and left external j ugular vein.Methods Adult male Wistar rats were randomly divided into three groups:anastomosis group (group A,n=15),ligation group (group B,n=15),normal group (group C,n=15).After feeding 3 months,hemodynamic index,pathological changes of myocardial tissue and lung biopsy,the plasma ET-1 con-centration,and the change of lung tissue endothelin-1 mRNA were determined.Results Compared with group B and C, the right ventricular systolic pressure increased significantly in group A.The concentration of plasma endothelin 1 was higher significantly in group A than group B and C (P<0.05).Fluorescence quantitative PCR results showed that the relative expression level of endothelin 1 mRNA in group A is 2.601 times of that in group B,3.373 times of that in group C,and that in group B is 1.297 times of that in the group C (P<0.05).Conclusion The arteriovenous shunt of pulmonary artery hypertension can be established successfully through anastomosis of the left common carotid artery and left external j ugular vein.Endothelin-1 is in a state of high expression in this model.